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Volume 9, Number 3—March 2003

Dispatch

Isolation of Escherichia coli O157:H7 from Intact Colon Fecal Samples of Swine1

Ingrid Feder*Comments to Author , F. Morgan Wallace*, Jeffrey T. Gray†, Pina Fratamico*, Paula J. Fedorka-Cray†, Rachel A. Pearce‡, Jeffrey E. Call*, Richard Perrine*, and John B. Luchansky*
Author affiliations: *U.S. Department of Agriculture, Wyndmoor, Pennsylvania, USA; †U.S. Department of Agriculture, Athens, Georgia, USA; ‡National Food Centre, Dublin, Ireland

Main Article

Table

Characterization of Escherichia coli O157:H7 and non-H7 isolates recovered from 305 swine fecal colon samplesa,b

Collection date Swine E. coli O157
isolate no. E. coli O157 positive colon samples/total colon samples collected Colon
ref.
no. No. of isolates recovered from sample PCR
characteristics E. coli O157 ribotyping PFGE type Antimicrobial resistance patternb
Feb. 16, 2001
1
2/5
1
1
rfbO157, fliCH7, stx1, eae, hly933
H7
1
Pan-sensitive

2

2
1
rfbO157, fliCH7, stx1, eae, hly933
H7
1
Pan-sensitive









Mar. 8, 2001
No isolates
0/20















Mar. 22, 2001
No isolates
0/40















Apr. 20, 2001
3
3/40
3
1
rfbO157
Non-H7
3
kan, strept, sulfa, tet

4

4
1
rfbO157
Non-H7
3
kan, strept, sulfa, tet

5

5
1
rfbO157
Non-H7
3
kan, sulfa, tet









May 4, 2001
6–11
4/50
6
6
rfbO157, fliCH7, stx1, stx2, eae
H7
2
Pan-sensitive

12–16

7
5
rfbO157, fliCH7, stx1, stx2, eae
H7
2
Pan-sensitive, except isolate no. 15 resistant to strept

17–25

8
9
rfbO157, fliCH7, stx1, stx2, eae
H7
2
Pan-sensitive

26,27

9
2
rfbO157, fliCH7, stx1, stx2, eae
H7
2
Pan-sensitive









May 16, 2001
28
9/50
10
1
rfbO157
Non-H7
4
Pan-sensitive

29

11
1
rfbO157
Non- H7
4
tet

30

12
1
rfbO157
Non-H7
4
tet

31

13
1
rfbO157
Non-H7
4
strept, tet

32

14
1
rfbO157
Non- H7
4
tet

33

15
1
rfbO157
Non-H7
4
tet

34

16
1
rfbO157
Non- H7
4
tet

35

17
1
rfbO157
Non- H7
4
tet

36

18
1
rfbO157
Non-H7
4
tet









June 20, 2001
No isolates
0/50















July 10, 2001
No isolates
0/50















Total=36 18/305 18

a Each isolate listed in the following table represents an isolate from an individual colon sample.
bFeb., February; Mar., March; Apr., April; ref., reference; PCR, polymerase chain reaction; PFGE, pulsed-field gel electrophoresis; kan, kanamycin; strept, streptomycin; sulfa, sulfamethoxazole; tet, tetracycline.

Main Article

1Presented in part at the 102nd Annual Meeting of the American Society of Microbiologists, Salt Lake City, Utah, May 2002.

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