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Volume 9, Number 5—May 2003

Research

Entamoeba moshkovskii Infections in Children in Bangladesh

Ibne Karin M. Ali*†1, Mohammad Bakhtiar Hossain†1, Shantanu Roy†, Patrick F. Ayeh-Kumi‡, William A. Petri§, Rashidul Haque†, and C. Graham Clark*Comments to Author 
Author affiliations: *London School of Hygiene and Tropical Medicine, London, England; †International Centre for Diarrheal Disease Research, Dhaka, Bangladesh; ‡University of Ghana Medical School, Accra, Ghana; §University of Virginia School of Medicine, Charlottesville, Virginia, USA; 1These authors contributed equally to this work.

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Figure 2

ArgTCT locus. ArgTCT sequences from Entamoeba histolytica HM-1:IMSS (GenBank accession no. AZ535059), E. dispar SAW760 (GenBank accession no. AF 525284), E. moshkovskii Laredo (GenBank accession no. AF 525285), and MS15-3646 (GenBank accession no. AF525286) were aligned at the 5´(A) and 3´ (B) ends to design E. moshkovskii–specific primers. The EmR primer sequences are shown in italic and bold with E. moshkovskii–specific positions underlined. C. Schematic representation of ArgTCT loci from E. h

Figure 2. ArgTCT locus. ArgTCT sequences from Entamoeba histolytica HM-1:IMSS (GenBank accession no. AZ535059), E. dispar SAW760 (GenBank accession no. AF 525284), E. moshkovskii Laredo (GenBank accession no. AF 525285), and MS15-3646 (GenBank accession no. AF525286) were aligned at the 5´(A) and 3´ (B) ends to design E. moshkovskii–specific primers. The EmR primer sequences are shown in italic and bold with E. moshkovskii–specific positions underlined. C. Schematic representation of ArgTCT loci from E. histolytica HM-1:IMSS, E. dispar SAW760, and E. moshkovskii. Locations of the primers used in polymerase chain reaction amplification are indicated by small arrows, the tRNA genes are indicated by large arrows, and the short tandem repeats by shaded boxes (not to scale.)

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