Emerging Infectious Disease ISSN: 1080-6059

Reemergence of Epidemic Vibrio cholerae O139, Bangladesh

Shah M. Faruque*, Nityananda Chowdhury*, M. Kamruzzaman*, Q. Shafi Ahmad*, A.S.G. Faruque*, M. Abdus Salam*, T. Ramamurthy†, G. Balakrish Nair*, Andrej Weintraub‡, and David A. Sack*

Author affiliations: *International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh; †National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India; ‡Karolinska Instute, Huddinge, Sweden

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Figure 3

Southern hybridization analysis of rstR genes in toxigenic Vibrio cholerae O139 strains isolated from the recent epidemic in Bangladesh (lanes 5–9) and in previously isolated O139 strains from 1992 to 1998 (lanes 1–4). Genomic DNA was digested with BglI and probed with the rstRET probe (A) and with the rstRCal probe (B). Numbers indicating molecular sizes of bands correspond to 1-kb DNA ladder (BRL) used as molecular size markers.

Figure 3. Southern hybridization analysis of rstR genes in toxigenic Vibrio cholerae O139 strains isolated from the recent epidemic in Bangladesh (lanes 5–9) and in previously isolated O139 strains from 1992 to 1998 (lanes 1–4). Genomic DNA was digested with BglI and probed with the rstR^ET probe (A) and with the rstR^Cal probe (B). Numbers indicating molecular sizes of bands correspond to 1-kb DNA ladder (BRL) used as molecular size markers.

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