Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 9, Number 9—September 2003

Research

Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome

Rickjason C. W. Chan*Comments to Author , John S. Tam*, Ching-Wan Lam*, Edward Chan*, Alan Wu*, Chi-Kong Li*, Thomas A. Buckley*, King-Cheung Ng*, Gavin M. Joynt*, Frankie W.T. Cheng*, Ka-Fai To*, Nelson Lee*, David S.C. Hui*, Jo L.K. Cheung*, Ida Chu*, Esther Liu*, Sydney S.C. Chung*, and Joseph J.Y. Sung*
Author affiliations: *Faculty of Medicine of the Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China

Main Article

Table

Distribution of human metapneumovirus reverse transcription–polymerase chain reaction results among 25 positive nasopharyngeal aspiratesa,b

No. of patients (%) Human metapneumovirus F-gene sequence detected by RT-PCR
Nasopharyngeal aspirate HEp-2 cells LLC-MK2 cells MDCK cells
1 (4.0)
Positive
Positive
Positive
Positive
1 (4.0)
Positive
Positive
Positive
Negative
1 (4.0)
Negative
Positive
Positive
Negative
1 (4.0)
Negative
Positive
Negative
Positive
18 (72.0)
Negative
Positive
Negative
Negative
3 (12.0) Negative Negative Positive Negative

aRT-PCR, reverse transcription-polymerase chain reaction; Hep-2, human laryngeal carcinoma monolayer; LLC-MK2, rhesus monkey kidney monolayer; MDCK, Mardin Darby Canine Kidney monolayer; BGM, Buffalo green monkey kidney monolayer.
bThe human metapneumovirus RT-PCR results for all human embryonic lung fibroblast, BGM and Vero cell cultures were negative.

Main Article

TOP