Volume 9, Number 9—September 2003
Research
Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome
Rickjason C. W. Chan*
, John S. Tam*, Ching-Wan Lam*, Edward Chan*, Alan Wu*, Chi-Kong Li*, Thomas A. Buckley*, King-Cheung Ng*, Gavin M. Joynt*, Frankie W.T. Cheng*, Ka-Fai To*, Nelson Lee*, David S.C. Hui*, Jo L.K. Cheung*, Ida Chu*, Esther Liu*, Sydney S.C. Chung*, and Joseph J.Y. Sung*
, John S. Tam*, Ching-Wan Lam*, Edward Chan*, Alan Wu*, Chi-Kong Li*, Thomas A. Buckley*, King-Cheung Ng*, Gavin M. Joynt*, Frankie W.T. Cheng*, Ka-Fai To*, Nelson Lee*, David S.C. Hui*, Jo L.K. Cheung*, Ida Chu*, Esther Liu*, Sydney S.C. Chung*, and Joseph J.Y. Sung*Table
Distribution of human metapneumovirus reverse transcription–polymerase chain reaction results among 25 positive nasopharyngeal aspiratesa,b
| No. of patients (%) | Human metapneumovirus F-gene sequence detected by RT-PCR |
|||
|---|---|---|---|---|
| Nasopharyngeal aspirate | HEp-2 cells | LLC-MK2 cells | MDCK cells | |
| 1 (4.0) |
Positive |
Positive |
Positive |
Positive |
| 1 (4.0) |
Positive |
Positive |
Positive |
Negative |
| 1 (4.0) |
Negative |
Positive |
Positive |
Negative |
| 1 (4.0) |
Negative |
Positive |
Negative |
Positive |
| 18 (72.0) |
Negative |
Positive |
Negative |
Negative |
| 3 (12.0) | Negative | Negative | Positive | Negative |
aRT-PCR, reverse transcription-polymerase chain reaction; Hep-2, human laryngeal carcinoma monolayer; LLC-MK2, rhesus monkey kidney monolayer; MDCK, Mardin Darby Canine Kidney monolayer; BGM, Buffalo green monkey kidney monolayer.
bThe human metapneumovirus RT-PCR results for all human embryonic lung fibroblast, BGM and Vero cell cultures were negative.
