Figure 3. . Analytical isoelectric focusing (IEF) of serially diluted broth induced enzymes. The isoelectric points (pIs) of the two prominent bands midway and at the bottom of the gel are 6.9 and >9.0. Lanes 1–3: uninduced enzyme serially diluted 1:1, 1:2, and 1:4. Lanes 4–6: enzyme induced with 8 μg/mL of cefoxitin serially diluted 1:1, 1:2, and 1:4. Lanes 7–9: enzyme induced with 4 μg/mL of imipenem serially diluted 1:1, 1:2 and 1:4. Lane 10: NOR-1 control, induced with 16 μg/mL of cefoxitin.
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