Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 13, Number 5—May 2007
Dispatch

Enteroaggregative Escherichia coli Related to Uropathogenic Clonal Group A

Faith Wallace-Gadsden*1, James R. Johnson†‡, John Wain§, and Iruka N. Okeke*Comments to Author 

Author affiliations: *Haverford College, Haverford, Pennsylvania, USA;; †Minneapolis Veterans Administration Medical Center, Minneapolis, Minnesota, USA;; ‡University of Minnesota, Minneapolis, Minnesota, USA; and; §Wellcome Trust Sanger Institute, Cambridgeshire, United Kingdom

Main Article

Table 2

Multilocus sequence types of CGA-associated fumC single-nucleotide polymorphism–positive strains*

Strain Clinical condition, country Allele profile
ST ST complex
adk fumC gyrB icd mdh purA recA
C08
Diarrhea, Nigeria
21
35
61
52
5
5
4
394
69
C14
Diarrhea, Nigeria
21
35
61
52
5
5
4
394
69
E23
Diarrhea, Nigeria
21
35
61
52
5
72
4
432
69
E30
Diarrhea, Nigeria
21
35
61
52
5
5
4
394
69
G10
Diarrhea, Nigeria
21
35
61
52
5
5
4
394
69
G17a
Diarrhea, Nigeria
21
35
61
52
5
5
4
394
69
G59
Diarrhea, Nigeria
21
35
61
52
5
5
4
394
69
G67b
Diarrhea, Nigeria
21
35
61
52
5
5
4
394
69
E64
Healthy, Nigeria
21
35
61
52
5
5
4
394
69
G108
Healthy, Nigeria
21
35
61
52
5
5
4
394
69
144-1
Diarrhea, Thailand
21
35
61
52
5
5
4
394
69
SEQ102†
UTI (CGA), USA
21
35
27
6
5
5
4
69
69
C16
Healthy, Nigeria
21
22
2
6
5
5
4
512
Unassigned
G55
Healthy, Nigeria
6
4
33
1
20
12
7
423
Unassigned
E56
Healthy, Nigeria
18
22
17
6
5
5
4
31
31
E62
Healthy, Nigeria
21
125
61
52
5
5
4
471
Unassigned
E68
Healthy, Nigeria
18
22
94
6
5
5
4
449
Unassigned
G103
Healthy, Nigeria
18
22
94
6
5
5
4
449
Unassigned
G121a
Healthy, Nigeria
18
22
1
6
5
5
4
474
Unassigned
G149 Healthy, Nigeria 18 22 1 6 5 5 4 474 Unassigned

*CGA, clonal group A; ST, sequence type; UTI, urinary tract infection.
†Data from reference (14). Data for all other isolates are from this study.

Main Article

References
  1. Czeczulin  JR, Whittam  TS, Henderson  IR, Navarro-Garcia  F, Nataro  JP. Phylogenetic analysis of virulence genes in enteroaggregative and diffusely adherent Escherichia coli. Infect Immun. 1999;67:26929.PubMedGoogle Scholar
  2. Okeke  IN, Scaletsky  IC, Soars  EH, Macfarlane  LR, Torres  AG. Molecular epidemiology of the iron utilization genes of enteroaggregative Escherichia coli. J Clin Microbiol. 2004;42:3644. DOIPubMedGoogle Scholar
  3. Okeke  IN, Lamikanra  A, Czeczulin  J, Dubovsky  F, Kaper  JB, Nataro  JP. Heterogeneous virulence of enteroaggregative Escherchia coli strains isolated from children in Southwest Nigeria. J Infect Dis. 2000;181:25260. DOIPubMedGoogle Scholar
  4. Fields  PI, Blom  K, Hughes  HJ, Helsel  LO, Feng  P, Swaminathan  B. Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM. J Clin Microbiol. 1997;35:106670.PubMedGoogle Scholar
  5. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk susceptibility tests, 8th Edition; Approved standard. Villanova (PA): The Committee; 2003. p. 130.
  6. Cerna  JF, Nataro  JP, Estrada-Garcia  T. Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains. J Clin Microbiol. 2003;41:213840. DOIPubMedGoogle Scholar
  7. Okeke  IN, Lamikanra  A, Steinruck  H, Kaper  JB. Characterization of Escherichia coli strains from cases of childhood diarrhea in provincial southwestern Nigeria. J Clin Microbiol. 2000;38:712.PubMedGoogle Scholar
  8. Johnson  JR, Manges  AR, O'Bryan  TT, Riley  LW. A disseminated multidrug-resistant clonal group of uropathogenic Escherichia coli in pyelonephritis. Lancet. 2002;359:224951. DOIPubMedGoogle Scholar
  9. Manges  AR, Johnson  JR, Foxman  B, O’Bryan  TT, Fullerton  KE, Riley  LW. Widespread distribution of urinary tract infections caused by a multidrug-resistant Escherichia coli clonal group. N Engl J Med. 2001;345:100713. DOIPubMedGoogle Scholar
  10. Manges  AR, Perdreau-Remington  F, Solberg  O, Riley  LW. Multidrug-resistant Escherichia coli clonal groups causing community-acquired bloodstream infections. J Infect. 2005;53:259. DOIPubMedGoogle Scholar
  11. Ramchandani  M, Manges  AR. DebRoy C, Smith SP, Johnson JR, Riley LW. Possible animal origin of human-associated, multidrug-resistant, uropathogenic Escherichia coli. Clin Infect Dis. 2005;40:2517. DOIPubMedGoogle Scholar
  12. Tartof  SY, Solberg  OD, Manges  AR, Riley  LW. Analysis of a uropathogenic Escherichia coli clonal group by multilocus sequence typing. J Clin Microbiol. 2005;43:58604. DOIPubMedGoogle Scholar
  13. Johnson  JR, Owens  K, Manges  AR, Riley  LW. Rapid and specific detection of Escherichia coli clonal group A by gene-specific PCR. J Clin Microbiol. 2004;42:261822. DOIPubMedGoogle Scholar
  14. Escobar-Paramo  P, Clermont  O, Blanc-Potard  AB, Bui  H, Le Bouguenec  C, Denamur  E. A specific genetic background is required for acquisition and expression of virulence factors in Escherichia coli. Mol Biol Evol. 2004;21:108594. DOIPubMedGoogle Scholar
  15. Wirth  T, Falush  D, Lan  R, Colles  F, Mensa  P, Wieler  LH, Sex and virulence in Escherichia coli: an evolutionary perspective. Mol Microbiol. 2006;60:113651. DOIPubMedGoogle Scholar

Main Article

1Current affiliation: Tufts University School of Medicine, Boston, Massachusetts, USA

Page created: June 23, 2010
Page updated: June 23, 2010
Page reviewed: June 23, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external