TY - JOUR AU - van den Hoogen, Bernadette G. AU - Herfst, Sander AU - Sprong, Leo AU - Cane, Patricia A. AU - Forleo-Neto, Eduardo AU - de Swart, Rik L. AU - Osterhaus, Albert D.M.E. AU - Fouchier, Ron A.M. T1 - Antigenic and Genetic Variability of Human Metapneumoviruses T2 - Emerging Infectious Disease journal PY - 2004 VL - 10 IS - 4 SP - 658 SN - 1080-6059 AB - Human metapneumovirus (HMPV) is a member of the subfamily Pneumovirinae within the family Paramyxoviridae. Other members of this subfamily, respiratory syncytial virus and avian pneumovirus, can be divided into subgroups based on genetic or antigenic differences or both. For HMPV, the existence of different genetic lineages has been described on the basis of variation in a limited set of available sequences. We address the antigenic relationship between genetic lineages in virus neutralization assays. In addition, we analyzed the genetic diversity of HMPV by phylogenetic analysis of sequences obtained for part of the fusion protein (n = 84) and the complete attachment protein open reading frames (n = 35). On the basis of sequence diversity between attachment protein genes and the differences in virus neutralization titers, two HMPV serotypes were defined. Each serotype could be divided into two genetic lineages, but these did not reflect major antigenic differences. KW - Paramyxoviridae KW - Pneumovirinae KW - human metapneumovirus KW - genetic variability KW - antigenic variability KW - serotypes KW - fusion protein KW - attachment protein KW - the Netherlands KW - Finland KW - England KW - Hong Kong KW - Brazil DO - 10.3201/eid1004.030393 UR - https://wwwnc.cdc.gov/eid/article/10/4/03-0393_article ER - End of Reference