TY - JOUR AU - Onyango, Clayton O. AU - Ofula, Victor O. AU - Sang, Rosemary C. AU - Konongoi, Samson L. AU - Sow, Abdourahmane AU - De Cock, Kevin M. AU - Tukei, Peter M. AU - Okoth, Fredrick A. AU - Swanepoel, Robert AU - Burt, Felicity J. AU - Waters, Norman C. AU - Coldren, Rodney L. T1 - Yellow Fever Outbreak, Imatong, Southern Sudan T2 - Emerging Infectious Disease journal PY - 2004 VL - 10 IS - 6 SP - 1064 SN - 1080-6059 AB - In May 2003, the World Health Organization received reports about a possible outbreak of a hemorrhagic disease of unknown cause in the Imatong Mountains of southern Sudan. Laboratory investigations were conducted on 28 serum samples collected from patients in the Imatong region. Serum samples from 13 patients were positive for immunoglobulin (Ig) M antibody to flavivirus, and serum samples from 5 patients were positive by reverse transcription–polymerase chain reaction with both the genus Flavivirus reactive primers and yellow fever virus–specific primers. Nucleotide sequencing of the amplicons obtained with the genus Flavivirus oligonucleotide primers confirmed yellow fever virus as the etiologic agent. Isolation attempts in newborn mice and Vero cells from the samples yielded virus isolates from five patients. Rapid and accurate laboratory diagnosis enabled an interagency emergency task force to initiate a targeted vaccination campaign to control the outbreak. KW - Yellow fever KW - ELISA KW - Oligonucleotide primer KW - amplicon KW - nucleotide sequence KW - Sudan KW - South Sudan DO - 10.3201/eid1006.030738 UR - https://wwwnc.cdc.gov/eid/article/10/6/03-0738_article ER - End of Reference