TY - JOUR AU - Nitsche, Andreas AU - Schweiger, Brunhilde AU - Ellerbrok, Heinz AU - Niedrig, Matthias AU - Pauli, Georg T1 - SARS Coronavirus Detection T2 - Emerging Infectious Disease journal PY - 2004 VL - 10 IS - 7 SP - 1300 SN - 1080-6059 AB - We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms. KW - coronavirus KW - SARS KW - real-time RT-PCR KW - molecular diagnostics DO - 10.3201/eid1007.030678 UR - https://wwwnc.cdc.gov/eid/article/10/7/03-0678_article ER - End of Reference