TY - JOUR AU - Colborn, James AU - Koita, Ousmane AU - Cissé, Ousmane AU - Bagayoko, Mamadou AU - Guthrie, Edward AU - Krogstad, Donald T1 - Identifying and Quantifying Genotypes in Polyclonal Infections due to Single Species T2 - Emerging Infectious Disease journal PY - 2006 VL - 12 IS - 3 SP - 475 SN - 1080-6059 AB - Simultaneous infection with multiple pathogens of the same species occurs with HIV, hepatitis C, Epstein-Barr virus, dengue, tuberculosis, and malaria. However, available methods do not distinguish among or quantify pathogen genotypes in individual patients; they also cannot test for novel insertions and deletions in genetically modified organisms. The strategy reported here accomplishes these goals with real-time polymerase chain reaction (PCR) and capillary electrophoresis. Real-time PCR with allotype-specific primers defines the allotypes (strains) present and the intensity of infection (copy number). Capillary electrophoresis defines the number of genotypes within each allotype and the intensity of infection by genotype. This strategy can be used to study the epidemiology of emerging infectious diseases with simultaneous infection by multiple genotypes, as demonstrated here with malaria. It also permits testing for insertions or deletions in genetically modified organisms that may be used for bioterrorism. KW - allotype KW - capillary electrophoresis KW - copy number KW - deletions KW - genotype KW - insertions KW - PCR KW - Mali DO - 10.3201/eid1203.050572 UR - https://wwwnc.cdc.gov/eid/article/12/3/05-0572_article ER - End of Reference