TY - JOUR AU - Wang, Jann-Yuan AU - Chou, Chien-Hong AU - Lee, Li-Na AU - Hsu, Hsiao-Leng AU - Jan, I-Shiow AU - Hsueh, Po-Ren AU - Kao, Chuan-Liang AU - Luh, Kwen-Tay T1 - Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ T2 - Emerging Infectious Disease journal PY - 2007 VL - 13 IS - 4 SP - 553 SN - 1080-6059 AB - We evaluated an enzyme-linked immunospot assay for interferon-γ (T SPOT-TB) for rapid diagnosis of active tuberculosis (TB) in a disease-endemic area. From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with TB were recruited, and a blood sample was obtained for T SPOT-TB assay within 7 days of microbiologic studies. Sixty-five patients were studied, including 39 (60%) with active TB. Thirty-five (53.8%) patients had underlying medical conditions. Thirty-seven patients had positive cultures for Mycobacterium tuberculosis, and 11 patients had positive cultures for nontuberculous mycobacteria. The sensitivity, specificity, positive predictive value, and negative predictive value of the T SPOT-TB assay were 87.2%, 88.5%, 91.9%, and 82.1%, respectively. The accuracy of this test in diagnosing active TB is >80%, even in an area with a high incidence of nontuberculous mycobacteria disease. KW - Tuberculosis KW - ELISPOT KW - latent infection KW - interferon-γ KW - research KW - Taiwan DO - 10.3201/eid1304.051195 UR - https://wwwnc.cdc.gov/eid/article/13/4/05-1195_article ER - End of Reference