TY - JOUR
AU - Delhaes, Laurence
AU - Harun, Azian
AU - Chen, Sharon C.A.
AU - Nguyen, Quoc
AU - Slavin, Monica
AU - Heath, Christopher H.
AU - Maszewska, Krystyna
AU - Halliday, Catriona
AU - Robert, Vincent
AU - Sorrell, Tania C.
AU - Meyer, Wieland
T1 - Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum
T2 - Emerging Infectious Disease journal
PY - 2008
VL - 14
IS - 2
SP - 282
SN - 1080-6059
AB - One hundred clinical isolates from a prospective nationwide study of scedosporiosis in Australia (2003–2005) and 46 additional isolates were genotyped by internal transcribed spacer–restriction fragment length polymorphism (ITS-RFLP) analysis, ITS sequencing, and M13 PCR fingerprinting. ITS-RFLP and PCR fingerprinting identified 3 distinct genetic groups. The first group corresponded to Scedosporium prolificans (n = 83), and the other 2 comprised isolates previously identified as S. apiospermum: one of these corresponded to S. apiospermum (n = 33) and the other to the newly described species S. aurantiacum (n = 30). Intraspecies variation was highest for S. apiospermum (58%), followed by S. prolificans (45%) and S. aurantiacum (28%) as determined by PCR fingerprinting. ITS sequence variation of 2.2% was observed among S. apiospermum isolates. No correlation was found between genotype of strains and their geographic origin, body site from which they were cultured, or colonization versus invasive disease. Twelve S. prolificans isolates from 2 suspected case clusters were examined by amplified fragment length polymorphism analysis. No specific clusters were confirmed.
KW - Scedosporium prolificans
KW - Scedosporium aurantiacum
KW - Scedosporium apiospermum
KW - molecular epidemiology
KW - ITS-sequencing
KW - ITS-RFLP
KW - PCR fingerprinting
KW - AFLP
KW - research
KW - Australia
DO - 10.3201/eid1402.070920
UR - https://wwwnc.cdc.gov/eid/article/14/2/07-0920_article
ER - End of Reference