TY - JOUR AU - Ujike, Makoto AU - Shimabukuro, Kozue AU - Mochizuki, Kiku AU - Obuchi, Masatsugu AU - Kageyama, Tsutomu AU - Shirakura, Masayuki AU - Kishida, Noriko AU - Yamashita, Kazuyo AU - Horikawa, Hiroshi AU - Kato, Yumiko AU - Fujita, Nobuyuki AU - Tashiro, Masato AU - Odagiri, Takato T1 - Oseltamivir-Resistant Influenza Viruses A (H1N1) during 2007–2009 Influenza Seasons, Japan T2 - Emerging Infectious Disease journal PY - 2010 VL - 16 IS - 6 SP - 926 SN - 1080-6059 AB - To monitor oseltamivir-resistant influenza viruses A (H1N1) (ORVs) with H275Y in neuraminidase (NA) in Japan during 2 influenza seasons, we analyzed 3,216 clinical samples by NA sequencing and/or NA inhibition assay. The total frequency of ORVs was 2.6% (45/1,734) during the 2007–08 season and 99.7% (1,477/1,482) during the 2008–09 season, indicating a marked increase in ORVs in Japan during 1 influenza season. The NA gene of ORVs in the 2007–08 season fell into 2 distinct lineages by D354G substitution, whereas that of ORVs in the 2008–09 season fell into 1 lineage. NA inhibition assay and M2 sequencing showed that almost all the ORVs were sensitive to zanamivir and amantadine. The hemagglutination inhibition test showed that ORVs were antigenetically similar to the 2008–09 vaccine strain A/Brisbane/59/2007. Our data indicate that the current vaccine or zanamivir and amantadine are effective against recent ORVs, but continuous surveillance remains necessary. KW - Viruses KW - influenza KW - oseltamivir KW - drug resistant KW - neuraminidase KW - influenza A (H1N1) KW - respiratory infections KW - Japan KW - research DO - 10.3201/eid1606.091623 UR - https://wwwnc.cdc.gov/eid/article/16/6/09-1623_article ER - End of Reference