TY - JOUR AU - Bialek-Davenet, Suzanne AU - Criscuolo, Alexis AU - Ailloud, Florent AU - Passet, Virginie AU - Jones, Louis AU - Delannoy-Vieillard, Anne-Sophie AU - Garin, Benoit AU - Le Hello, Simon AU - Arlet, Guillaume AU - Nicolas-Chanoine, Marie-Hélène AU - Decré, Dominique AU - Brisse, Sylvain T1 - Genomic Definition of Hypervirulent and Multidrug-Resistant Klebsiella pneumoniae Clonal Groups T2 - Emerging Infectious Disease journal PY - 2014 VL - 20 IS - 11 SP - 1812 SN - 1080-6059 AB - Multidrug-resistant and highly virulent Klebsiella pneumoniae isolates are emerging, but the clonal groups (CGs) corresponding to these high-risk strains have remained imprecisely defined. We aimed to identify K. pneumoniae CGs on the basis of genome-wide sequence variation and to provide a simple bioinformatics tool to extract virulence and resistance gene data from genomic data. We sequenced 48 K. pneumoniae isolates, mostly of serotypes K1 and K2, and compared the genomes with 119 publicly available genomes. A total of 694 highly conserved genes were included in a core-genome multilocus sequence typing scheme, and cluster analysis of the data enabled precise definition of globally distributed hypervirulent and multidrug-resistant CGs. In addition, we created a freely accessible database, BIGSdb-Kp, to enable rapid extraction of medically and epidemiologically relevant information from genomic sequences of K. pneumoniae. Although drug-resistant and virulent K. pneumoniae populations were largely nonoverlapping, isolates with combined virulence and resistance features were detected. KW - Klebsiella pneumoniae KW - hypervirulent KW - invasive infections KW - multidrug resistance KW - clonal groups KW - emerging clones KW - genome evolution KW - multilocus sequence typing KW - MLST KW - diagnostics KW - high-throughput sequencing KW - bacteria DO - 10.3201/eid2011.140206 UR - https://wwwnc.cdc.gov/eid/article/20/11/14-0206_article ER - End of Reference