TY - JOUR
AU - Arya, Lalan Kumar
AU - Rathinam, Sivakumar
AU - Lalitha, Prajna
AU - Kim, Usha
AU - Ghatani, Sudeep
AU - Tandon, Veena
T1 - Trematode Fluke Procerovum varium as Cause of Ocular Inflammation in Children, South India
T2 - Emerging Infectious Disease journal
PY - 2016
VL - 22
IS - 2
SP - 192
SN - 1080-6059
AB - Trematodes are recognized as a group of emerging parasites in tropical countries. We identified a trematode as a cause of ocular granulomas that developed in children who bathed in ponds or rivers in South India. DNA was isolated from patients’ surgically excised granulomas and from the trematode cercariae (larvae) released by the snail Melanoides tuberculata in water in which the children bathed. Real-time and conventional PCRs were performed that targeted ribosomal DNA regions spanning the internal transcribed spacer 2 and 28S sequences of this trematode. The PCR-amplified products were subjected to bidirectional sequencing. Analysis of sequences for the granuloma samples and the trematode cercariae showed maximum sequence similarity with Procerovum varium (family Heterophyidae). Our results confirmed the etiology of the ocular infection, implicating snail vectors as environmental risk factors for ocular parasitosis.
KW - granuloma
KW - uveitis
KW - trematode
KW - cercaria
KW - real-time PCR
KW - sequencing
KW - ribosomal DNA
KW - eye disease
KW - child
KW - fresh water
KW - lakes
KW - ponds
KW - rivers
KW - parasites
KW - India
DO - 10.3201/eid2202.150051
UR - https://wwwnc.cdc.gov/eid/article/22/2/15-0051_article
ER - End of Reference