Preliminary Favorable Outcome for Medically and Surgically Managed Extensively Drug-Resistant Tuberculosis, France, 2009–2014

We report 20 cases of extensively drug-resistant tuberculosis managed in France. Treatment was individualized and included bedaquiline and linezolid for most patients and surgery in 8 patients. At last follow-up (22 months), 19 patients had achieved conversion from positive to negative on culture testing. These promising results of comprehensive management obtained in a small series deserve confirmation.

M ultidrug-resistant tuberculosis (TB) and extensively drug-resistant TB (XDR TB) are among the most difficult infections to treat and are major public health concerns worldwide (1). In France, the number of imported XDR TB cases has dramatically increase recently, especially cases originating from countries of the former Union of Soviet Socialist Republics (2).

The Study
During 2009-2014, we identified 20 persons who were admitted to 2 tertiary-care hospitals in Rennes and Paris, France, with culture-positive XDR TB infections. Patients were identified through hospital database searches; patient data were extracted from medical charts. For each patient, we performed direct examination of sputum smears; cultures on Lowenstein-Jensen medium; genotypic resistance profiling (GenoType MTBDR Plus; HAIN Lifescience, Nehren, Germany); and in vitro drug susceptibility testing (DST) on Lowenstein-Jensen medium, according to the proportions method.
All patients were isolated in negative-pressure rooms until their respiratory sample culture results converted from positive to negative (hereafter referred to as culture conversion). Medical and surgical therapeutic options were determined during multidisciplinary meetings involving infectious diseases, respiratory diseases, microbiology, and thoracic surgery departments. In agreement with World Health Organization guidelines (3), we selected anti-TB drug therapy on the basis of results from previously used agents or genotypic and phenotypic DST. Throughout hospitalization, treatment toxicity was carefully monitored through clinical assessments, routine laboratory tests, therapeutic drug monitoring, audiograms for patients on aminoglycosides, and weekly electrocardiograms. Treatment efficacy was monitored through thoracic imaging and monthly examination of respiratory samples. For patients with nondisseminated pulmonary TB, surgery was considered at the initiation of medical treatment if success of the treatment was deemed unlikely because of extensive lesions or after 3 months of optimized medical treatment if sputum conversion was not achieved. In agreement with procedures implemented by the French Information Protection Commission, all data were anonymized and collected on a standardized form.
During the intensive phase of treatment, each patient was given 4-9 presumably active anti-TB agents ( Table  2). All patients required long-term central venous access for administration of amikacin or carbapenems. Duration of medical treatment was individualized but continued a minimum of 12 months after culture conversion. Median duration of treatment was 24 (range 18-51) months. Median length of stay in the acute-care setting was 67 (IQR 55-124) days.
Thoracic surgery was performed in 8 patients (lobectomy in 5, pneumonectomy in 3), of whom 2 had severe postsurgical complications. Six of these patients were culture-positive before surgery, and their cultures converted a median of 25 days postsurgery (IQR 20-33 days).
After a median follow-up of 22 (IQR 15-27) months, 18 patients were alive; 2 had died of causes considered unrelated to TB. Sputum cultures had converted for 19 patients, 4 of whom had completed treatment and a median of 38 (IQR 29-40) months of posttreatment follow-up. Median time from treatment initiation to culture conversion was 100 (IQR 75-114) days. One patient is still under treatment and has not experienced culture conversion. No patients were lost to follow-up.

Conclusions
This small series of XDR TB cases managed in a highincome country illustrates the potential safety and efficacy of multidisciplinary and individualized treatment with a multidrug regimen that includes new anti-TB agents (e.g., bedaquiline); innovative use of older agents (e.g., linezolid); and the combination of imipenem plus amoxicillin/clavulanate. The series also emphasizes difficulties faced by healthcare professionals caring for XDR TB patients.
The final outcome could not be ascertained for most study patients because they are still receiving antimycobacterial therapy; however, the 90% survival rate after a median follow-up of 22 months after treatment initiation is reassuring and compares favorably with survival rates of 66%, 54%, and 38% in the United States (4), South Africa (5), and the United Kingdom (6), respectively. The high rate of microbiologic conversion in our study (95%) also reflects the potentially achievable treatment efficacy, even in the context of previously treated XDR TB cases.
Our findings should be interpreted cautiously because of the small number of patients and the relatively short follow-up at the time of this writing. However, the overall figures of culture conversion are more satisfactory than those previously reported in high-income countries, where conversion rates ranged from 46.7% to 76.1% (4,6-10), and in high-prevalence settings, where conversion rates are lower (5,11,12). Preliminary results from a study conducted in South Africa were more favorable: samples from 48 (76%) of 63 patients with 6 months follow-up were culture-negative (13). Postsurgery sputum conversion was rapidly achieved for patients in our study; thus, pulmonary resection surgery, although risky, may also have contributed substantially to treatment successes. Numerous difficulties were encountered during the study. Patients were referred to our centers soon after arriving in France, causing communication difficulties for patients with a limited understanding of French and English. Medical histories were long and complex, and most patients were in advanced stages of pulmonary TB.
The numerous side effects observed during prolonged anti-TB regimens must be optimally and intensively managed; otherwise, patients may not complete treatment. Because of the limited number of potentially active anti-TB agents, drugs with documented long-term toxicities must also be included in multidrug regimens. The high incidence of breakthrough candidemia cases (5/20 patients [25%]) was not anticipated, although prolonged exposure to broad-spectrum antimicrobial drugs and long-term central venous access are acknowledged risk factors for candidemia. This risk must be taken into account when considering treatment of XDR TB with carbapenems and amoxicillin/clavulanate. The overall good tolerability of linezolid may be a result of the low dosage (routinely, 600 mg/d initially, decreased to 300 mg/d if toxicity is suspected, even with limited evidence).
Financial, social, and cultural aspects of the management of vulnerable and marginalized patients are also essential and time-consuming. Prolonged hospital stays were necessary for patients in our study, resulting in high healthcare costs, as previously reported in South Africa (14). Limited resources and vulnerability are risk factors for noncompliance and disease progression. However, failure to adequately address these issues would translate into additional XDR TB transmission in the community and increased illness and death, which could result in a much higher societal burden. Previous experience in high-income countries has documented that comprehensive care of TB patients is cost-effective, even in the most vulnerable and marginalized populations, especially when multidrug-resistant or XDR TB are involved (15).
Our results reflect the situation in a high-income setting with free access to all potentially active drugs, extensive investigation of responsible strains (e.g., using DST and genotypic tests), daily monitoring of adverse events, regular multidisciplinary meetings to tailor treatment to any new event and evaluate the need for thoracic surgery in selected cases, dedicated medical and paramedical staff, and psychosocial support. Unfortunately, the situation may not be the same in the countries most affected by XDR TB.   B.H. received fees from Janssen Pharmaceutica for a roundtable on multidrug-resistant tuberculosis. P.T. received fees for participation in review activities and support for travel to meetings from Janssen-Cilag.