TY - JOUR AU - Kim, Won-Keun AU - No, Jin Sun AU - Lee, Seung-Ho AU - Song, Dong Hyun AU - Lee, Daesang AU - Kim, Jeong-Ah AU - Gu, Se Hun AU - Park, Sunhye AU - Jeong, Seong Tae AU - Kim, Heung-Chul AU - Klein, Terry AU - Wiley, Michael AU - Palacios, Gustavo AU - Song, Jin-Won T1 - Multiplex PCR−Based Next-Generation Sequencing and Global Diversity of Seoul Virus in Humans and Rats T2 - Emerging Infectious Disease journal PY - 2018 VL - 24 IS - 2 SP - 249 SN - 1080-6059 AB - Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR–based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of R. norvegicus rats in South Korea during 2000–2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges. KW - next-generation sequencing KW - multiplex PCR KW - orthohantavirus KW - Seoul virus KW - hantavirus KW - viruses KW - phylogenetic analysis KW - global diversity KW - humans KW - rats KW - South Korea KW - reverse transcription PCR KW - RT-PCR KW - real-time quantitative PCR DO - 10.3201/eid2402.171216 UR - https://wwwnc.cdc.gov/eid/article/24/2/17-1216_article ER - End of Reference