TY - JOUR AU - Lutgring, Joseph AU - Zhu, Wenming AU - de Man, Tom J.B. AU - Avillan, Johannetsy AU - Anderson, Karen AU - Lonsway, David AU - Rowe, Lori AU - Batra, Dhwani AU - Rasheed, J. Kamile AU - Limbago, Brandi T1 - Phenotypic and Genotypic Characterization of Enterobacteriaceae Producing Oxacillinase-48–Like Carbapenemases, United States T2 - Emerging Infectious Disease journal PY - 2018 VL - 24 IS - 4 SP - 700 SN - 1080-6059 AB - Oxacillinase (OXA)–48–like carbapenemases remain relatively uncommon in the United States. We performed phenotypic and genotypic characterization of 30 Enterobacteriaceae producing OXA-48–like carbapenemases that were recovered from patients during 2010–2014. Isolates were collected from 12 states and not associated with outbreaks, although we could not exclude limited local transmission. The alleles β-lactamase OXA-181 (blaOXA-181) (43%), blaOXA-232 (33%), and blaOXA-48 (23%) were found. All isolates were resistant to ertapenem and showed positive results for the ertapenem and meropenem modified Hodge test and the modified carbapenem inactivation method; 73% showed a positive result for the Carba Nordmann–Poirel test. Whole-genome sequencing identified extended-spectrum β-lactamase genes in 93% of isolates. In all blaOXA-232 isolates, the gene was on a ColKP3 plasmid. A total of 12 of 13 isolates harboring blaOXA-181 contained the insertion sequence ΔISEcp1. In all isolates with blaOXA-48, the gene was located on a TN1999 transposon; these isolates also carried IncL/M plasmids. KW - phenotypic characterization KW - genotypic characterization KW - oxacillinase-48–like carbapenemases KW - OXA-48 KW - beta-lactamases KW - antimicrobial resistance KW - Enterobacteriaceae KW - bacteria KW - anti-bacterial agents KW - United States DO - 10.3201/eid2404.171377 UR - https://wwwnc.cdc.gov/eid/article/24/4/17-1377_article ER - End of Reference