Influenza D Virus Circulation in Cattle and Swine, Luxembourg, 2012–2016

We detected antibodies against influenza D in 80.2% of the cattle sampled in Luxembourg in 2016, suggesting widespread virus circulation throughout the country. In swine, seroprevalence of influenza D was low but increased from 0% to 5.9% from 2012 to 2014–2015.

I nfluenza D virus (IDV), a new orthomyxovirus distantly related to influenza C virus, was described in pigs with respiratory symptoms in 2011 (1). Although mild symptoms only were reported in experimental pig and calf infections, the virus has been implicated in bovine respiratory disease complex (1)(2)(3). Cattle are currently considered the main host of the virus, but other livestock species are also susceptible (4). In Europe, IDV circulation has been reported in France (5), Italy (6,7), and Ireland (8). Recent serosurveys in Italy showed extremely high seroprevalence rates in cattle (92.4% seropositive) (9) and a low but increasing seroprevalence in swine, from 0.6% in 2009 to 11.7% in 2015 (7). We investigated the presence of IDV in cattle and swine farms in Luxembourg.
We found an overall seroprevalence of 80.2% in cattle (361/450; HI titer range 20-1,280) (online Technical Appendix Figure); 97.7% of herds (43/44) had >1 seropositive animal. Average within-farm seroprevalence was 83.0% (range 20%-100%; Figure, panel A). These results suggested that IDV affects most animals in nearly all farms (Figure, panel A). All animals were much older than 6 months (average 70.5 mo, range 23-209 mo), so it is unlikely that the antibodies were maternally derived (10). The median age of seropositive animals (61 months) was significantly higher than the median age of seronegative animals (41 months; p<0.001). Seroprevalence was higher in beef cattle (88.0%, 95/108) than in dairy cattle (75.6%, 133/176; meat or dairy production type was not known for 166 animals), but beef cattle were also on average older than dairy cattle. A binary logistic regression model including herd as a random effect and age and production type as fixed effects revealed that only age substantially affected IDV seropositivity.
Most of the cattle investigated were born in Luxembourg (90%, 405/450), but IDV antibodies were found regardless of country of birth (others were born in Germany, France, Belgium, and Italy). This information demonstrates that our results cannot be explained by importation of seropositive animals alone and that IDV transmission takes place in Luxembourg. Within-herd seroprevalence of cattle herds was similar for herds located near the borders as well as those further inland, suggesting that the virus could also spread to and from the neighboring countries (Belgium, France, and Germany), for example, through cross-border grazing.
In Luxembourg, IDV seroprevalence was low in swine compared with cattle but has increased during recent years (0% in 2012 to 5.9% [17/287] in 2014-2015), as it has in Italy (7). We detected seropositive animals in 6/29 (20.7%) swine herds (Figure, panel B). The low virus prevalence from nasal swabs (0% in 2009, 0.7% [3/427] in 2014-2015) and the low viral RNA concentration (9.7-94.5 copies/μL) were not conducive to amplification of genetic material for sequencing. The low levels of virus circulation in pigs shown by seroprevalence data, the absence of symptoms at the time of sampling (3), and the short shedding period under experimental infection (1) probably contributed to the low detection rates observed in swine nasal swab samples. The IDV RNA-positive nasal swab samples originated from 2 different herds, 1 of which was also seropositive (9/10 pigs with HI titer >20; no samples were available from the second herd). Although we collected all 3 PCR-positive samples on the same day at the same slaughterhouse, it is unlikely that the pigs were infected during their short stay there.
Taken together, our results suggested that IDV circulates widely throughout cattle farms in Luxembourg and can be considered hyperenzootic in the country. Once introduced into a herd, IDV seems to spread very efficiently, given the high within-farm seroprevalence rates. In light of cross-border trade and grazing, the region beyond Luxembourg's borders may be also hyperenzootic for IDV. Although IDV mainly affects cattle, we detected IDV antibodies in pigs and an increased seroprevalence in pig herds. We are planning systematic serologic and virologic screenings along with epidemiologic surveys to investigate the genetic diversity of IDV strains in Luxembourg, to evaluate the effect of IDV infection on cattle and pig health and productivity, and to study IDV interaction with other pathogens.