TY - JOUR AU - Costantini, Veronica AU - Morantz, Esther AU - Browne, Hannah AU - Ettayebi, Khalil AU - Zeng, Xi-Lei AU - Atmar, Robert AU - Estes, Mary AU - Vinjé, Jan T1 - Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation T2 - Emerging Infectious Disease journal PY - 2018 VL - 24 IS - 8 SP - 1453 SN - 1080-6059 AB - Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies. KW - norovirus KW - human intestinal enteroids KW - inactivation KW - viruses KW - replication KW - virus inactivation DO - 10.3201/eid2408.180126 UR - https://wwwnc.cdc.gov/eid/article/24/8/18-0126_article ER - End of Reference