TY - JOUR AU - Okba, Nisreen M.A. AU - Widjaja, Ivy AU - Li, Wentao AU - GeurtsvanKessel, Corine AU - Farag, Elmoubasher A.B.A. AU - Al-Hajri, Mohammed AU - Park, Wan Beom AU - Oh, Myoung-don AU - Reusken, Chantal B.E.M. AU - Koopmans, Marion P.G. AU - Bosch, Berend-Jan AU - Haagmans, Bart T1 - Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies T2 - Emerging Infectious Disease journal PY - 2020 VL - 26 IS - 5 SP - 1024 SN - 1080-6059 AB - We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome. KW - Middle East respiratory syndrome coronavirus KW - MERS KW - neutralization KW - hemagglutination KW - lumazine synthase KW - antibodies KW - human KW - dromedary KW - serology KW - nanoparticle KW - spike KW - viruses KW - zoonoses KW - respiratory infections KW - MERS-CoV KW - Middle East respiratory syndrome DO - 10.3201/eid2605.190921 UR - https://wwwnc.cdc.gov/eid/article/26/5/19-0921_article ER - End of Reference