TY - JOUR AU - Caron, Yannick AU - Bory, Sotharith AU - Pluot, Michel AU - Nheb, Mary AU - Chan, Sarin AU - Prum, Sang Houn AU - Lim, Sun Bun Hong AU - Sim, Mala AU - Sengdoeurn, Yi AU - Sovann, Ly AU - Khieu, Virak AU - Vallée, Isabelle AU - Yera, Hélène T1 - Human Outbreak of Trichinellosis Caused by Trichinella papuae Nematodes, Central Kampong Thom Province, Cambodia T2 - Emerging Infectious Disease journal PY - 2020 VL - 26 IS - 8 SP - 1759 SN - 1080-6059 AB - In September 2017, a severe trichinellosis outbreak occurred in Cambodia after persons consumed raw wild pig meat; 33 persons were infected and 8 died. We collected and analyzed the medical records for 25 patients. Clinical signs and symptoms included myalgia, facial or peripheral edema, asthenia, and fever. We observed increased levels of creatine phosphokinase and aspartate aminotransferase­, as well as eosinophilia. Histopathologic examination of muscle biopsy specimens showed nonencapsulated Trichinella larvae. A Trichinella excretory/secretory antigen ELISA identified Trichinella IgM and IgG. Biopsy samples were digested and larvae were isolated and counted. PCR for the 5S rDNA intergenic spacer region and a multiplex PCR, followed by sequencing identified the parasite as Trichinella papuae. This species was identified in Papua New Guinea during 1999 and in several outbreaks in humans in Thailand. Thus, we identified T. papuae nematodes in humans in Cambodia. KW - Trichinella papuae KW - trichinellosis KW - nematodes KW - parasites KW - outbreak KW - food safety KW - biopsy KW - serologic analysis KW - central Kampong Thom Province KW - Cambodia KW - rDNA intergenic spacer region KW - Kampong Thom Province DO - 10.3201/eid2608.191497 UR - https://wwwnc.cdc.gov/eid/article/26/8/19-1497_article ER - End of Reference