TY - JOUR AU - Zhao, Shan AU - Schuurman, Nancy AU - Li, Wentao AU - Wang, Chunyan AU - Smit, Lidwien A.M. AU - Broens, Els AU - Wagenaar, Jaap AU - van Kuppeveld, Frank J.M. AU - Bosch, Berend-Jan AU - Egberink, Herman T1 - Serologic Screening of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Cats and Dogs during First Coronavirus Disease Wave, the Netherlands T2 - Emerging Infectious Disease journal PY - 2021 VL - 27 IS - 5 SP - 1362 SN - 1080-6059 AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect many animal species, including minks, cats, and dogs. To gain insights into SARS-CoV-2 infections in cats and dogs, we developed and validated a set of serologic assays, including ELISA and virus neutralization. Evaluation of samples from animals before they acquired coronavirus disease and samples from cats roaming SARS-CoV-2–positive mink farms confirmed the suitability of these assays for specific antibody detection. Furthermore, our findings exclude SARS-CoV-2 nucleocapsid protein as an antigen for serologic screening of cat and dog samples. We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April–May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive. Although seroprevalence in cats and dogs that had unknown SARS-CoV-2 exposure was low during the first coronavirus disease wave, our data stress the need for development of continuous serosurveillance for SARS-CoV-2 in these 2 animal species. KW - severe acute respiratory syndrome coronavirus 2 KW - SARS-CoV-2 KW - coronaviruses KW - viruses KW - coronavirus disease KW - COVID-19 KW - respiratory infections KW - cats KW - dogs KW - serologic screening KW - ELISA KW - virus neutralization KW - receptor-binding domain KW - nucleocapsid protein KW - zoonoses KW - the Netherlands DO - 10.3201/eid2705.204055 UR - https://wwwnc.cdc.gov/eid/article/27/5/20-4055_article ER - End of Reference