Bedaquiline Resistance after Effective Treatment of Multidrug-Resistant Tuberculosis, Namibia

Bedaquiline is currently a key drug for treating multidrug-resistant or rifampin-resistant tuberculosis. We report and discuss the unusual development of resistance to bedaquiline in a teenager in Namibia, despite an optimal background regimen and adherence. The report highlights the risk for bedaquiline resistance development and the need for rapid drug-resistance testing.

We ensured adherence by inpatient directly observed treatment.Clinical and microbiological response was slow; culture and smear microscopy results were negative once after 5 months of treatment (Figure 2, panel C).However, culture reversion occurred, and sustained culture conversion was never achieved despite clinical and transient radiologic improvement (Figure 1).Subsequent molecular DST based on targeted nextgeneration sequencing documented several frame shift mutations in Rv0678 at position 779127 with 3.5% variant frequency, at 779130 with 16.0% frequency, and at 779407 with 27.8% frequency.Mutations in rpoB, katG and embB remained unchanged to baseline molecular DST.The de novo mutations were associated with phenotypic resistance to bedaquiline and clofazimine.All other drugs tested remained susceptible in molecular and phenotypic DST (Figure 2, panel B).We stopped bedaquiline and clofazimine administration and added 3 drugs, amikacin, meropenem/amoxicillin/clavulanic acid, and pretomanid, to maximize the probability of achieving conversion and cure.Treatment was ongoing as of February 2024.

Conclusions
Bedaquiline has been shown to be a key drug for improving outcomes in MDR/RR TB patients (4).However, recent studies have demonstrated the emergence of bedaquiline resistance in patients failing MDR TB treatment, which, at the population level, points toward rapid bedaquiline resistance evolution and spread (3,5).Our results are particularly alarming because we demonstrated the evolution of bedaquiline resistance despite the use of an effective background regimen and well-documented adherence to treatment.This result is in line with the findings of recently published work from Mozambique, in which Barilar et al. demonstrated that bedaquiline resistance was found not only in M. tuberculosis strains resistant to fluoroquinolones but also in MDR or RR M. tuberculosis strains susceptible to other drugs used in the BPaL(M) regimen (3).
Taking all evidence together, the data suggest that current MDR/RR TB treatment regimens are unable to prevent the development of bedaquiline resistance in a subset of patients.A specific combination of pharmacokinetic and pharmacodynamic properties of the drug and pathogen or patient markers potentially result in rapid resistance development.Detecting 3 different Rv0678 variants in the patient sample analyzed, an observation also made for several bedaquiline-resistant strains found previously (3,6), supports this observation.
In general, bedaquiline and clofazimine cross-resistance can result from underlying pretreatment resistance by infection with an already resistant strain, presence of heteroresistant strains and clonal populations, and de novo evolution of resistance during treatment (5).Here we demonstrate the rapid evolution and selection of several bedaquiline resistant subpopulations, despite resistance-appropriate treatment with 6 effective drugs.Our findings suggest a high bedaquiline resistance mutation rate that enables parallel emergence of different bedaquiline-resistant populations with different Rv0678 mutations in a given patient.That finding is also supported by large-scale sequencing data obtained from patients with bedaquiline resistance in Mozambique (3).
In a recent meta-analysis, Mallik et al. reported acquired phenotypic bedaquiline resistance in 2.2%, genotypic resistance in 4.4% of cases ( 5), whereas Perumal et al. reported phenotypic resistance in 2.1% of cases (7).Future studies should further investigate mechanisms of bedaquiline resistance development, for example, to identify patients at risk.Bedaqui- line seems to have a delayed bactericidal response, which could be a risk factor for drug resistance developing during early treatment, particularly in extensive disease (8).Some studies suggested the use of highly bactericidal companion drugs in combination with bedaquiline.Van Deun et al. reviewed the regimen composition on the basis of the concept of core drugs and companion drugs (9).In accordance with this strategy, our patient received 2 core drugs (bedaquiline and levofloxacin), 1 highly bactericidal companion drug (linezolid) and 2 highly sterilizing drugs (pyrazinamide and clofazimine); we added cycloserine.Despite strictly following van Deun's concept of effective regimen composition, resistance to bedaquiline/clofazimine developed in the patient discussed here, who had severe lung destruction and high bacterial load.Derendinger et al. described acquired bedaquiline resistance in routine care in South Africa and considered use of <4 effective drugs, fluoroquinolone resistance, and previous or concurrent clofazimine use as risk factors for bedaquiline resistance (6).None of those factors were present in the case we describe.Shao et al. showed in their population pharmocokinetic model that the current WHO recommended dosing of bedaquiline achieves a probability >90% of target attainment (10) for bedaquiline, whereas the half-life of bacterial clearance was longer in pre-extensively drug-resistant (XDR) and XDR TB than in MDR TB.One might speculate that dose adjustments to bedaquiline favorably influence treatment outcomes (11), as has been done so far in individual cases applying therapeutic drug monitoring (12).
In conclusion, this case demonstrates the rapid evolution of phenotypic and genotypic resistance to bedaquiline and clofazimine, despite an effective individualized regimen.This finding is alarming because the BPaL(M) regimen may not be completely effective in an unknown proportion of patients.In particular, cases of extensive disease might be associated with a high risk for resistance and failure in realworld scenarios.We recommend further research into mechanisms of resistance, and prevention thereof, as well as rapid scale-up of DST capacity to identify and properly treat such cases as quickly as possible.

Figure 1 .
Figure 1.Chest radiographs showing lungs of tuberculosis patient in Namibia who had bedaquiline resistance develop.A) At treatment initiation; B) at culture conversion; C) 3 months after conversion.