TY - JOUR AU - Ong, Corinne S. L. AU - Eisler, Diane L. AU - Alikhani, Alireza AU - Fung, Vicki W. K. AU - Tomblin, Joan AU - Bowie, William R. AU - Isaac-Renton, Judith L. T1 - Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype T2 - Emerging Infectious Disease journal PY - 2002 VL - 8 IS - 3 SP - 263 SN - 1080-6059 AB - In this study, we genotyped parasites from the fecal specimens of sporadic cryptosporidiosis cases in British Columbia from 1995 to 1999. Genotyping was conducted by polymerase chain amplification of the internal transcribed spacer region, a hypervariable region in the 18S rRNA gene and the Cryptosporidium oocyst wall protein gene. Subsequent analysis was by restriction fragment length polymorphism and DNA sequencing. We identified two new Cryptosporidium genotypes in humans. One of these genotypes has been found recently in deer in New York state. The other genotype has not been identified in humans or animals. These results have important implications for drinking water quality strategies, especially for communities that obtain drinking water supplies from surface sources located in forested regions with deer populations. KW - Cryptosporidium KW - cryptosporidiosis KW - molecular KW - genotype KW - polymerase chain reaction KW - restriction fragment length polymorphism KW - 18S rRNA KW - sequencing KW - Canada DO - 10.3201/eid0803.010194 UR - https://wwwnc.cdc.gov/eid/article/8/3/01-0194_article ER - End of Reference