TY - JOUR AU - Trindade, Giliane de Souza AU - Guimarães da Fonseca, Flávio AU - Marques, João Trindade AU - Nogueira, Maurício Lacerda AU - Mendes, Luiz Claudio Nogueira AU - Borges, Alexandre Secorun AU - Peiró, Juliana Regina AU - Pituco, Edviges Maristela AU - Bonjardim, Cláudio Antônio AU - Ferreira, Paulo César Peregrino AU - Lima, Maurício Teixeira T1 - Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle T2 - Emerging Infectious Disease journal PY - 2003 VL - 9 IS - 2 SP - 155 SN - 1080-6059 AB - We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus. KW - Araçatuba virus KW - cowpoxlike outbreak KW - emergent orthopoxvirus KW - TK KW - VGF KW - HA KW - and ATI genes KW - research KW - Brazil DO - 10.3201/eid0902.020244 UR - https://wwwnc.cdc.gov/eid/article/9/2/02-0244_article ER - End of Reference