TY - JOUR AU - Chan, Rickjason C. W. AU - Tam, John S. AU - Lam, Ching-Wan AU - Chan, Edward AU - Wu, Alan AU - Li, Chi-Kong AU - Buckley, Thomas A. AU - Ng, King-Cheung AU - Joynt, Gavin M. AU - Cheng, Frankie W.T. AU - To, Ka-Fai AU - Lee, Nelson AU - Hui, David S.C. AU - Cheung, Jo L.K. AU - Chu, Ida AU - Liu, Esther AU - Chung, Sydney S.C. AU - Sung, Joseph J.Y. T1 - Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome T2 - Emerging Infectious Disease journal PY - 2003 VL - 9 IS - 9 SP - 1058 SN - 1080-6059 AB - We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS. KW - Metapneumovirus KW - coronavirus KW - severe acute respiratory syndrome KW - SARS KW - virus isolation KW - Hong Kong KW - China DO - 10.3201/eid0909.030304 UR - https://wwwnc.cdc.gov/eid/article/9/9/03-0304_article ER - End of Reference