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Volume 10, Number 10—October 2004
Letter

Boiling and Bacillus Spores

Eugene W. Rice*Comments to Author , Laura J. Rose†, Clifford H. Johnson*, Laura A. Boczek*, Matthew J. Arduino†, and Donald J. Reasoner*
Author affiliations: *U.S. Environmental Protection Agency, Cincinnati, Ohio, USA; †U.S. Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Table

Inactivation of Bacillus spp. by boiling in tap water

Initial log10 CFU/mL
Boiling timesa log10 CFU/mL
1 Min
3 Min
5 Min
Organism Covered Uncovered Covered Uncovered Covered Uncovered Covered Uncovered
B. anthracis Sterne 4.95 4.92 0.11 NDb <0c 2.13 <0c 2.01
B. cereus (commercial) 4.62 4.59 0.81 1.94 <0c 1.50 <0c 1.46
B. cereus, ATCC 9592 4.54 4.76 <0c 0.78 <0c 0.60 <0c 0.48
B. thuringiensis ATCC 35646 4.63 4.46 <0c 1.76 <0c 1.58 <0c 1.47

aValues are means of duplicate experiments <0.25 log units.
bND, not determined.
c<0, a number reading below the detection level.

Main Article

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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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