Early Release

Synopses

• Morphologic and Molecular Identification of Human Ocular Infection Caused by Pelecitus species, Thailand
  P. Rujkorakarn et al.
• Clinical Significance, Species Distribution, and Temporal Trends of Nontuberculous Mycobacteria, Denmark, 1991–2022
  V. Dahl et al.

Research

• Clinical Aspects and Disease Severity of Streptococcus dysgalactiae Subspecies equisimilis Bacteremia, Finland
  V. Nevanlinna et al.
• Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes
  C. Rafferty et al. View Abstract

  Spread of the Anopheles stephensi mosquito, an invasive malaria vector, threatens to put an additional 126 million persons per year in Africa at risk for malaria. To accelerate the early detection and rapid response to this mosquito species, confirming its presence and geographic extent is critical. However, existing molecular species assays require specialized laboratory equipment, interpretation, and sequencing confirmation. We developed and optimized a colorimetric rapid loop-mediated isothermal amplification assay for molecular An. stephensi species identification. The assay requires only a heat source and reagents and can be used with or without DNA extraction, resulting in positive color change in 30–35 minutes. We validated the assay against existing PCR techniques and found 100% specificity and analytical sensitivity down to 0.0003 nanograms of genomic DNA. The assay can successfully amplify single mosquito legs. Initial testing on samples from Marsabit, Kenya, illustrate its potential as an early vector detection and malaria mitigation tool.

• Formation of Single and Multispecies Biofilm by Isolates from Septic Transfusion Reactions in Platelet Bag Model
  C. Hapip et al.
• Role of Direct Sexual Contact in Human Transmission of Monkeypox Virus
  G. Sberna et al.
• Molecular Epidemiology of Western Equine Encephalitis Virus, South America, 2023–2024
  A. Campos et al.

Dispatches

• Co-Circulation of Genetically Distinct High Pathogenicity Avian Influenza A(H5N5) and (H5N1) Viruses in Crows, Hokkaido, Japan
  Y. Hew et al.
• Mosquitoes as Vectors of Buruli Ulcer Based on Transmission of Ross River/Barmah Forest Virus and Mycobacterium ulcerans, Australia
  A. H. Buultjens et al.
• Emergence of Extensively Drug-Resistant Neisseria gonorrhoeae, France, 2023
  F. Caméléna et al.
• Fatal Case of Naegleria fowleri Primary Amebic Meningoencephalitis from Indoor Surfing Center, Taiwan, 2023
  H. Wei et al.
• Avian and Human Influenza A Virus Receptors in Bovine Mammary Gland
  C. Kristensen et al.
• Zoonotic Mansonella ozzardi in Raccoons, 2019–2022, Costa Rica
  J. Quesada et al.
• Participatory, Virologic, and Wastewater Surveillance Data to Assess Underestimation of COVID-19 Incidence, Germany, 2020–2024
  A. Loenenbach et al.

Photo Quiz

• Photo Quiz
  A. Cucu et al.

Research Letters

• Molecular Confirmation of Taenia solium Taeniasis in Child, Timor-Leste
  H. Jin et al.
• Emerging Leishmania donovani Lineages Associated with Cutaneous Leishmaniasis, Himachal Pradesh, India, 2023
  P. Lypaczewski et al.
• Confirmed Case of Autochthonous Human Babesiosis, Hungary
  D. Sipos et al.

In Memoriam

• In Memoriam: Thomas J. Gryczan (1949–2024)
  B. Breedlove and B. Segal

Research

• Age- and Gender-Specific Differences in Lyme Disease Health-Related Behaviors, Ontario, Canada, 2015–2022
  J. A. Adams et al.

Synopsis

• Role of Department of Defense Global Emerging Infections Surveillance Program in Pandemic Preparedness and Response, United States
  K. Creppage et al.

Research

• Molecular Surveillance of Multidrug-Resistant Bacteria among Refugees from Afghanistan in 2 US Military Hospitals during Operation Allies Refuge, 2021
  C. Anderson et al.