Figure. RealArt HPA-Coronavirus LightCycler (RealArt HPA Coronavirus RT-PCR) reverse transcription-polymerase chain reaction (PCR) Assay results. PCR results from 5 μL RNA are displayed in channel F1/F2 of the LightCycler instrument (A). Four quantification standards are included in the assay to generate a standard curve (B). An internal control, added at the RNA isolation stage, is used to monitor both the quality of the RNA isolation as well as possible PCR inhibition (C).
1Drs. Mazzulli and Farcas contributed equally to the manuscript. All authors jointly conceived and designed the study and wrote the report. Gabriella A. Farcas performed the majority of the reverse transcription-polymerase chain reaction assays.
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