Volume 10, Number 10—October 2004
Research
Fluoroquinolone Resistance in Penicillin-resistant Streptococcus pneumoniae Clones, Spain
Table 3
PFGE | Strain | Serotype | Resistance pattern | aa and nt changes in QRDR |
||
---|---|---|---|---|---|---|
ParC | ParE | GyrA | ||||
Referent | R6 | NT | S | None | None | None |
Spain23F-1 | CipR-8 | 23F | PEClTCSxTCp | G128 (GGT), K137N, S79F | I460V, I476 (ATT) | Y75 (TAT) |
CipR-5 | 19Ab | PTCSxTCp | G128 (GGT), K137N, S79F | I460V, I476 (ATT) | Y75 (TAT) | |
CipR-9 | 23F | PEClTCSxTCp | G128 (GGT), K137N, S79Y | I460V, I476 (ATT) | Y75 (TAT) | |
CipR-12 | 23F | PTCCp | G128 (GGT), K137N, D83G | I460V, I476 (ATT) | Y75 (TAT) | |
CipR-30, -31, -32, -33 | 23F | PEClTCSxTCp | G128 (GGT), K137N, S79F | I460V, I476 (ATT) | Y75 (TAT), S81F | |
CipR-48, -49 | 23F | PEClTCSxTCp | G128 (GGT), K137N, S79F | I460V, I476 (ATT) | Y75 (TAT), E85K | |
CipR-73 |
19Ab |
EClTSxTCp |
S79F |
None |
Y75 (TAT), S81F |
|
Spain14-5 | CipR-24 | 14 | PEClTCSxTCp | S79F | None | Y75 (TAT), S81F |
CipR-41 | 14 | PEClTCSxTCp | S79F | None | Y75 (TAT), S81Y | |
CipR-55 | 14 | PEClTCSxTCp | S79Y | None | Y75 (TAT), S81F | |
CipR-66, -67 | 14 | PEClTCSxTCp | S79F | D435N | Y75 (TAT), E85K | |
CipR-38, -39, -40 |
14 |
PEClTCSxTCp |
S79F |
None |
Y75 (TAT), V88I, S81Y |
|
Spain9V-3 | CipR-10 | 9V | PSxTCp | K137N, S79Y | I460V | Y75 (TAT) |
CipR-14 | 14b | PESxTCp | K137N, S79A | I460V | Y75 (TAT), S81F | |
CipR-51 | 14b | PSxTCp | K137N, S79Y | I460V | Y75 (TAT), S81F | |
CipR-20 | 9V | PSxTCp | K137N, S79F | I460V | Y75 (TAT), S81F | |
CipR-28 | 19Fb | PSxTCp | K137N, S79F | I460V | Y75 (TAT), S81F | |
CipR-58 |
14b |
PSxTCp |
K137N, D83N |
I460V |
Y75 (TAT), S81F |
|
Spain6B-2 | CipR-3 | 6B | PEClTSxTCp | S79F | None | Y75 (TAT) |
CipR-17, -18, -19 | 6B | PEClTCSxTCp | S79F | None | Y75 (TAT), S81F | |
CipR-72 |
6B |
PEClTSxTCp |
S79F |
None |
Y75 (TAT), S81F |
|
Sweden15A-25 | CipR-4 | 15A | PEClTCp | G77 (GGA), S79F | I460V | Y75 (TAT) |
CipR-64 | 15A | PEClTCp | G77 (GGA) | I460V, D435N | Y75 (TAT), S81Y | |
CipR-60 | 15A | PEClTCp | G77 (GGA), D83Y | I460V | Y75 (TAT), E85K | |
CipR-45, -50 |
15A |
PEClTCp |
G77 (GGA), S79F |
I460V |
Y75 (TAT), E85K |
|
C | CipR-59 | 19F | PTCSxTCp | D83N | None | Y75 (TAT), S81F |
CipR-43, -44 | 19F | PTCSxTCp | S79F | None | Y75 (TAT), S81Y | |
CipR-65 |
19F |
PEClTCSxTCp |
S79Y, D83N |
None |
Y75 (TAT), E85K |
|
D | CipR-57 | 19F | PEClTCp | G77 (GGA), S79Y | I460V, P454S | Y75 (TAT), S81Y |
CipR-29 | 19F | PEClTCCp | G77 (GGA), S79F | I460V | Y75 (TAT), S81F | |
CipR-46, -47 |
19F |
PEClTCp |
G77 (GGA), S79F |
I460V |
Y75 (TAT), E85K |
|
A | CipR-1 | 3 | Cp | R95C, S79F | I460V | Y75 (TAT), H104 (CAC) |
CipR-2 | 3 | Cp | R95C, S79F | None | Y75 (TAT), H104 (CAC) | |
CipR-15 |
3 |
TCCp |
R95C, S79F |
None |
Y75 (TAT), H104 (CAC), S81F |
|
B | CipR-61 | 3 | Cp | None | I460V, D435N | Y75 (TAT), S81F |
CipR-52 | 3 | ECp | S79Y | I460V | Y75 (TAT), S81F |
aQRDR, quinolone-resistance determining regions; PFGE, pulse-field gel electrophoresis SmaI patterns; aa, amino acid; nt, nucleotide; S, susceptible to all antibiotics tested; P, resistant to penicillin (MIC 0.12–4 μg/mL); T, resistant to tetracycline (MIC >4 μg/mL); C, resistant to chloramphenicol (MIC >8 μg/mL); E, resistant to erythromycin (MIC >0.5 μg/mL); Cl, resistant to clindamycin (MIC >0.5 μg/mL); SxT, resistant to trimethropin-sulfamethoxazole (MIC >4/76 μg/mL); Cp, resistant to ciprofloxacin (MIC >4 μg/mL). Residue changes involved in fluoroquinolone resistance are showed in boldface, and double underlining indicates that the residue is located in a gene with a mosaic structure. A, PFGE type related to serotype 3 with MLST 260; B, PFGE type related to serotype 3 with MLST 180; NT, non typeable.
bCapsular switching.
1Spanish Pneumococcal Infection Study Network—general coordination: Román Pallarés; participants and centers: Ernesto García (Centro de Investigaciones Biológicas, Madrid); Julio Casal, Asuncion Fenoll, Adela G. de la Campa (Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid); Emilio Bouza, (Hospital Gregorio Marañon, Madrid); Fernando Baquero (Hospital Ramón y Cajal, Madrid); Francisco Soriano, José Prieto (Fundación Jiménez Díaz y Hospital Clínico, Madrid); Román Pallarés, Josefina Liñares (Hospital Universitari de Bellvitge, Barcelona); Javier Garau, Javier Martínez Lacasa (Hospital Mutua de Terrassa, Barcelona); Cristina Latorre (Hospital Sant Joan de Deu, Barcelona); Emilio Pérez-Trallero, Alberto González (Hospital Donostia, San Sebastian); Juan García de Lomas (Hospital Clínico, Valencia); and Ana Fleites (Hospital Central de Asturias).