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Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition
Laboratory Study

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery*, Dean D. Erdman*Comments to Author , Michael D. Bowen*, Bruce R. Newton*, Jonas M. Winchell*, Richard F. Meyer*, Suxiang Tong*, Byron T. Cook*, Brian P. Holloway*, Karen A. McCaustland*, Paul A. Rota*, Bettina Bankamp*, Luis E. Lowe*, Tom G. Ksiazek*, William J. Bellini*, and Larry J. Anderson*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Table 5

Results of real-time RT-PCR assay with specimens from patients with laboratory-confirmed SARS-CoV infectiona

Case ID Location Specimen ID Specimen Serology Vero E6
culture Conventional
RT-PCRb Real-time RT-PCR CTc values
SARS1 SARS2 SARS3 RNase P
05078
Toronto
2003756523
Lung, RM
N/A

+
24.2
21.6
23
23.9


2003756525
Lung, RU


+
24.9
21.5
23
23.7
05077
Thailand
2003756502
Throat swab
+
+
+
37.5
36.2
39.8
29.3
05000
Hong Kong
2003757035
Lung, RU
+

+
26.7
22.6
24.1
24.7


2003757036
Lung, LU


+
27.2
24.9
26.5
26


2003757037
Lung, RM


+
34.9
37.5
31.9
27.4


2003757038
Lung, LL


+
29.6
27
28.6
24.5
00220
Utah, USA
2003757508
Sputum
+
+
+
24.7
23
24.8
30.6
05001
Vietnam
2003757190
Throat wash
+
+
+
23.7
22.4
24.1
30.1
05008
Vietnam
2003757229
Throat wash
+

+
35.5
35.5
36.7
30
05010
Vietnam
2003757239
Throat wash
+

+
31.1
29.3
31.5
34.2
05013
Vietnam
2003757251
Throat wash
+

+
29.5
28.4
30.3
28.8
05017
Vietnam
2003757268
Throat wash
+
+
+
26
24.7
26.4
27.9
05316 Vietnam 2003759760 Throat swab N/A + N/A 25 25.3 28.2 28

aRT-PCR, reverse transcription–polymerase chain reaction; SARS-CoV, severe acute respiratory syndrome–associated coronavirus; CT, threshold cycle number; RM, right middle; RU, right upper; LU, left upper; LL, left lower; N/A, not applicable.
bRef. 5.
cValues shown mean of duplicate values.

Main Article

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Main Article

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Page updated: January 27, 2011
Page reviewed: January 27, 2011
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