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Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition

Laboratory Study

Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003

Junhui Zhai*1, Thomas Briese†1, Erhei Dai*, Xiaoyi Wang*, Xin Pang*, Zongmin Du*, Haihong Liu*, Jin Wang*, Hongxia Wang*, Zhaobiao Guo*, Zeliang Chen*, Lingxiao Jiang*, Dongsheng Zhou*, Yanping Han*, Omar Jabado†, Gustavo Palacios†, W. Ian Lipkin†Comments to Author , and Ruifu Yang*Comments to Author 
Author affiliations: *Institute of Microbiology and Epidemiology, Beijing, China; †Mailman School of Public Health of Columbia University, New York, New York, USA; 1Junhui Zhai and Thomas Briese contributed equally to the manuscript.

Main Article

Table

Summary of clinical samplesa

Specimens Total patients 1–10 d
11–20 d
21–30 d
31–40 d
>40 d
pos neg pos neg pos neg pos neg pos neg
Feces PCR
326
10
27
19
52
12
65
12
55
7
67
Blood PCR
426
28
34
20
21
22
143
26
132
NA
NA
Blood IgG
426
6
56
10
31
82
83
138
20
NA
NA
Blood IgM 426 8 54 6 35 63 102 82 76 NA NA

apos, positive;eg, negative; PCR, polymerase chain reaction; Ig, immunoglobulin; NA, not available.

Main Article

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