Figure 1. Molecular subtyping of representative Francisella tularensis isolates from Groups A, B, C, E, and F prairie dogs. The expected size PCR fragments for F. tularensis subsp. tularensis (Type A) and holarctica (Type B) are shown in lanes 1 and 2, respectively. Subtyping results for the five groups (A, B, C, E, F) are shown in lanes 3–9. Lane 3: TX021935 (A); lane 4: TX022151 (A); lane 5: TX022537 (B); lane 6: TX022592 (B); lane 7: TX022799 (C); lane 8: TX022107 (E); lane 9: CZ024233 (F). Lane 10: no DNA template control. Lane M: molecular weight markers.