Volume 10, Number 7—July 2004
Dispatch
Emergence of Multidrug-resistant Salmonella Paratyphi B dT+, Canada
Michael R. Mulvey*
, David Boyd*, Axel Cloeckaert†, Rafiq Ahmed*, Lai-King Ng, and the Provincial Public Health Laboratories1
, David Boyd*, Axel Cloeckaert†, Rafiq Ahmed*, Lai-King Ng, and the Provincial Public Health Laboratories1Table
PCR primer to detect various regions of SGI1
| Set | Primer | Primer sequence (5′ to 3′) | Coordinatesa | Product size (bp) |
|---|---|---|---|---|
| St1 |
U9-L1
P1-R1 |
TACTACAAGCAGATAACGCC
TAGAAACGACAAAGCGCGTG |
2771–2790
3660–3679 |
909 |
| St3 |
P134-L1
P134-R2 |
AATCGACACGCGCTGTATTG
CTTCCCATAATGCCGCAATG |
16350–16369
17287–17306 |
957 |
| St4 |
P134-L1
P134-R1 |
TGACCCAATTCCAAAGCCAC
GTGTTTGGGCAAGATCCCAG |
16784–16803
17820–17839 |
1490 |
| St5 |
St2-GP21
St2-GP6 |
ATAACGGCAGGTTCCGGTTC
CGATGAAGCGCACAAATTTG |
20173–20192
21089–21108 |
936 |
| St6 | St2-GP24 St2-GP28 | TCAAGATTCCTATCTGCAGG AGAGTTACTAGACCAAGCGC | 24363–24382 25182–25201 | 838 |
aCoordinates from accession no. AF261825.
1Provincial Public Health Laboratory Members: Lewis Abbott (Prince Edward Island), Kevin Forward (Nova Scotia), Glenna Hardy (New Brunswick), Greg Horsman (Saskatchewan), Judy Isaac-Renton (British Columbia), Frances Jamieson (Ontario), Jean Joly (Quebec), Jutta K. Preiksaitis (Alberta), Sam Ratnam (Newfoundland), Paul Van Caeseele (Manitoba).
