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Volume 10, Number 7—July 2004

Research

Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis

Wei-Kung Wang*†, Shey-Ying Chen†1, I-Jung Liu*1, Yee-Chun Chen†, Hui-Ling Chen*, Chao-Fu Yang*, Pei-Jer Chen*, Shiou-Hwei Yeh‡, Chuan-Liang Kao*, Li-Min Huang†, Po-Ren Hsueh†, Jann-Tay Wang†, Wang-Hwei Sheng†, Chi-Tai Fang†, Chien-Ching Hung†, Szu-Min Hsieh†, Chan-Ping Su†, Wen-Chu Chiang†, Jyh-Yuan Yang§, Jih-Hui Lin§, Szu-Chia Hsieh*, Hsien-Ping Hu*, Yu-Ping Chiang*, Jin-Town Wang*, Pan-Chyr Yang†, Shan-Chwen Chang†Comments to Author , and members of the SARS Research Group of the National Taiwan UniversityNational Taiwan University Hospital

Author affiliations: *National Taiwan University, Taipei, Taiwan; †National Taiwan University Hospital, Taipei, Taiwan; ‡National Health Research Institute, Taipei, Taiwan; §Center for Disease Control, Department of Health, Taipei, Taiwan; 1S.-Y. Chen and I-J. Liu contributed equally to the work.

Main Article

Figure 2

Detection of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in the epithelial cells in throat wash from SARS patients by an indirect immunofluorescence assay. (A,B) Spot slides of SARS-CoV-infected Vero E6 cells were incubated with the preimmune (A) or postimmune (B) serum from a rabbit immunized with the recombinant nucleocapsid protein of the SARS-CoV, followed by fluorescein isothiocyanate–conjugated goat anti-rabbit immunoglobulin G. Panels A and B demonstrate the sp

Figure 2. Detection of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in the epithelial cells in throat wash from SARS patients by an indirect immunofluorescence assay. (A,B) Spot slides of SARS-CoV-infected Vero E6 cells were incubated with the preimmune (A) or postimmune (B) serum from a rabbit immunized with the recombinant nucleocapsid protein of the SARS-CoV, followed by fluorescein isothiocyanate–conjugated goat anti-rabbit immunoglobulin G. Panels A and B demonstrate the specificity of the reagents. (C to G) Epithelial cells in throat wash from a healthy control (E) and two SARS patients, ID17 (C,D) and ID11 (F,G), were incubated with the preimmune (C,F) or postimmune (D,E,G) rabbit serum. (H) The light microscopic picture of (G), taken with the fluorescent light on.

Main Article

1S.-Y. Chen and I.-J. Liu contributed equally to the work.

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