Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis
Wei-Kung Wang*†, Shey-Ying Chen†1, I-Jung Liu*1, Yee-Chun Chen†, Hui-Ling Chen*, Chao-Fu Yang*, Pei-Jer Chen*, Shiou-Hwei Yeh‡, Chuan-Liang Kao*, Li-Min Huang†, Po-Ren Hsueh†, Jann-Tay Wang†, Wang-Hwei Sheng†, Chi-Tai Fang†, Chien-Ching Hung†, Szu-Min Hsieh†, Chan-Ping Su†, Wen-Chu Chiang†, Jyh-Yuan Yang§, Jih-Hui Lin§, Szu-Chia Hsieh*, Hsien-Ping Hu*, Yu-Ping Chiang*, Jin-Town Wang*, Pan-Chyr Yang†, Shan-Chwen Chang† , and members of the SARS Research Group of the National Taiwan UniversityNational Taiwan University Hospital
Author affiliations: *National Taiwan University, Taipei, Taiwan; †National Taiwan University Hospital, Taipei, Taiwan; ‡National Health Research Institute, Taipei, Taiwan; §Center for Disease Control, Department of Health, Taipei, Taiwan; 1S.-Y. Chen and I-J. Liu contributed equally to the work.
Figure 2. Detection of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in the epithelial cells in throat wash from SARS patients by an indirect immunofluorescence assay. (A,B) Spot slides of SARS-CoV-infected Vero E6 cells were incubated with the preimmune (A) or postimmune (B) serum from a rabbit immunized with the recombinant nucleocapsid protein of the SARS-CoV, followed by fluorescein isothiocyanate–conjugated goat anti-rabbit immunoglobulin G. Panels A and B demonstrate the specificity of the reagents. (C to G) Epithelial cells in throat wash from a healthy control (E) and two SARS patients, ID17 (C,D) and ID11 (F,G), were incubated with the preimmune (C,F) or postimmune (D,E,G) rabbit serum. (H) The light microscopic picture of (G), taken with the fluorescent light on.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.