Anucha Apisarnthanarak* , Rungrueng Kitphati†, Kanokporn Thongphubeth*, Prisana Patoomanunt*, Pimjai Anthanont*, Wattana Auwanit†, Pranee Thawatsupha†, Malinee Chittaganpitch†, Siriphan Saeng-Aroon†, Sunthareeya Waicharoen†, Piyaporn Apisarnthanarak‡, Gregory A. Storch§, Linda M. Mundy§, and Victoria J. Fraser§
Author affiliations: *Thammasart University Hospital, Pratumthani, Thailand; †National Institute of Health, Nonthaburi, Thailand; ‡Siriraj Hospital, Bangkok, Thailand; §Washington University School of Medicine, St. Louis, Missouri, USA
Figure 2. A reverse transcription–polymerase chain reaction (RT-PCR) specific for H5 gene band (358 bp) of avian influenza H5N1 that was recovered from our patient from nasopharyngeal aspirates by using H5-1/H5-2 primer. Lane A, molecular standard; lane B, H5 band isolated from our patient (358 bp); lane C, negative control; lane D, positive control. B. RT-PCR specific for H5 gene band (229 bp) of avian influenza (H5N1) that was recovered from our patient from nasopharyngeal aspiration by using H5-1456/H5-1685 primer. Lane A, molecular standard; lane B, positive control; lane C, H5 band isolated from our patient (229 bp).
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