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Volume 10, Number 8—August 2004

Perspective

Genomic-scale Analysis of Bacterial Gene and Protein Expression in the Host

John D. Boyce*Comments to Author , Paul A. Cullen*, and Ben Adler*
Author affiliations: *Monash University, Melbourne, Victoria, Australia

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Table

Large-scale measurement of bacterial gene or protein expression during real infections or interactions with host cells

Species Experiment type Description Reference
Growth in the host
Borrelia burgdorferi DNA microarray Growth in dialysis membranes implanted in rats 18
DNA microarray Adaptation to growth in dialysis membranes in rats 19
DNA microarray Alteration of lipoprotein expression during host adaptation in mice 20
Antigenic profiling Alteration of antigenic profile during host adaptation in mice 21
Pasteurella multocida DNA microarray Gene expression during growth in blood of infected chickens 15
DNA microarray Gene expression during growth in livers of infected chickens 14
Vibrio cholerae DNA microarray Gene expression during growth in rabbit ileal loops 16
DNA microarray Gene expression in rice water stools 17
Interaction with tissue culture cells
Chlamydia pneumoniae 2-dimensional gel electrophoresis Protein expression during growth in HEp-2 cells in response to interferon-γ 22
Neisseria meningitidis DNA microarray Interaction with epithelial and endothelial cells 24
DNA microarray Interaction with epithelial cells 23

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