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Volume 10, Number 9—September 2004

Research

Viral Loads in Clinical Specimens and SARS Manifestations

I.F.N. Hung*, V.C.C. Cheng*, A.K.L. Wu†, B.S.F. Tang*, K.H. Chan*, C.M. Chu‡, M.M.L. Wong§, W.T. Hui*, L.L.M. Poon*, D.M.W. Tse§, K.S. Chan‡, P.C.Y. Woo*, S.K.P. Lau*, J.S.M. Peiris*, and K.Y. Yuen*Comments to Author 
Author affiliations: *Queen Mary Hospital at the University of Hong Kong, Hong Kong Special Administrative Region (SAR), People’s Republic of China; †Prince of Wales Hospital, Hong Kong SAR, People’s Republic of China; ‡United Christian Hospital, Hong Kong SAR, People’s Republic of China; §Caritas Medical Centre, Hong Kong SAR, People’s Republic of China

Main Article

Table 5

Correlation of RT-qPCR clinical specimens taken during days 10 to 15, and clinical manifestations in 40 patients with SARS who had specimens taken from all four anatomical sitesPaP

Specimen Diarrhea
OB2B desaturation
Mechanical ventilation
Death
Y N p value; 
OR (CI) Y N p value; OR (CI) Y N p value; OR (CI) Y N p value; OR (CI)
NPA (n=40)
RT-qPCR (+) (n = 18) 11 7 0.4 11 7 0.02; 5.3 
(1.4–21.1) 7 11 < 0.01; 1.6 (1.1–2.4) 8 10 < 0.01; 1.8 (1.2–2.7)
RT-qPCR (–) (n = 22) 10 12 5 17 0 22 0 22
Median viral load (logB10B copies/mL)
2.9
0
0.4
5.1
0
< 0.01
6.4
0
< 0.01
6.4
0
< 0.01
Serum (n = 40)
RT-qPCR (+) (n = 18) 12 6 0.1 11 7 0.02; 5.3 
(1.4–21.1) 7 11 < 0.01; 1.6 (1.1–2.4) 8 10 < 0.01; 1.8 (1.2–2.7)
RT-qPCR (–) (n = 22) 9 13 5 17 0 22 0 22
Median viral load 
 (logB10B copies/mL)
2.3
0
0.3
2.4
0
0.02
2.6
0
< 0.01
2.6
0
< 0.01
Stool (n = 40)
RT-qPCR (+) (n = 30) 20 10 < 0.01; 18 (2.0–16.3) 12 18 1 5 25 1 7 23 0.7
RT-qPCR (–) (n = 10) 1 9 4 6 2 8 1 9
Median viral load 
 (logB10B copies/mL)
7.8
2.6
< 0.01
7.3
7.2
0.5
8.2
7.2
0.5
8.7
7
0.03
Urine (n = 40)
RT-qPCR (+) (n = 9) 6 3 0.5 3 6 0.7 1 8 1 2 7 1
RT-qPCR (–) (n = 31) 15 16 13 18 6 25 6 25
Median viral load 
 (logB10B copies/mL) 0 0 0.4 0 0 0.6 0 0 0.5 0 0 1

PaPRT-qPCR, quantitative reverse transcription–polymerase chain reaction; SARS, severe acute respiratory syndrome; Y, yes; N, no; OR, odds ratio; CI, 95% confidence interval; NPA, nasopharyngeal aspirates.

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