Volume 11, Number 2—February 2005
Research
Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea
Yeon-Joo Choi*1, Won-Jong Jang1*, Jong-Hyun Kim*, Ji-Sun Ryu*, Seung-Hyun Lee*, Kyung-Hee Park*, Hyung-Suk Paik†, Young-Sang Koh‡, Myung-Sik Choi§, and Ik-Sang Kim§
Table 1
Oligonucleotide primers for amplification of partial rickettsial genes*
| Primer | Target rickettsia group | Gene | Position | Nucleotide sequence (5′–3′) |
|---|---|---|---|---|
| rompB OF | SFG and TG | rompB† | 3,620–3,643 | GTAACCGGAAGTAATCGTTTCGTAA |
| rompB OR | SFG and TG | rompB | 4,131–4,109 | GCTTTATAACCAGCTAAACCACC |
| rompB SFG IF | SFG | rompB | 3,652–3,674 | GTTTAATACGTGCTGCTAACCAA |
| rompB SFG/TG IR | SFG and TG | rompB | 4,077–4,057 | GGTTTGGCCCATATACCATAAG |
| rompB TG IF | TG | rompB | 3,828–3,850 | AAGATCCTTCTGATGTTGCAACA |
| RpCS.877p§ | SFG and TG | gltA‡ | 877–895 | GGGGGCCTGCTCACGGCGG |
| RpCS.1,258n§ | SFG and TG | gltA | 1,258–1,237 | ATTGCAAAAAGTACAGTGAACA |
| RpCS.896p | SFG and TG | gltA | 896–915 | GGCTAATGAAGCAGTGATAA |
| RpCS.1,233n | SFG and TG | gltA | 1,233–1,215 | GCGACGGTATACCCATAGC |
*SFG, spotted fever group; TG, typhus group; OR, outer reverse primer; OF, outer forward primer.
†Oligonucleotide primer sequences derived from Rickettsia conorii genes (accession no. AF123721).
‡Oligonucleotide primer sequences derived from R. prowazekii genes (accession no. M17149).
§Primer sequences derived from (9).
