Emerging Infectious Disease ISSN: 1080-6059

Isolation of Waddlia malaysiensis, A Novel Intracellular Bacterium, from Fruit Bat (Eonycteris spelaea)

Paul K.B. Chua*†, John E. Corkill*, Poh Sim. Hooi‡, Soo Choon Cheng‡, Craig Winstanley*, and C. Anthony Hart*

Author affiliations: *University of Liverpool, Liverpool, United Kingdom; †National Public Health Laboratory, Kuala Lumpur, Malaysia; ‡University of Malaya, Kuala Lumpur, Malaysia

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Figure 1

A, Negative stain electronmicrograph of Waddlia malaysiensis elementary bodies. B–D, Thin-section electronmicrographs of cells infected with W. malaysiensis. B, large inclusion with elementary(e) and reticulate(r) bodies in HEK cells 72 h postinfection. C, a large inclusion in EBV-transformed human B-lymphocytes. D, dividing reticulate bodies in HEK cells 48 h postinfection in an inclusion with numerous surrounding mitochondria (arrow).

Figure 1. A, Negative stain electronmicrograph of Waddlia malaysiensis elementary bodies. B–D, Thin-section electronmicrographs of cells infected with W. malaysiensis. B, large inclusion with elementary(e) and reticulate(r) bodies in HEK cells 72 h postinfection. C, a large inclusion in EBV-transformed human B-lymphocytes. D, dividing reticulate bodies in HEK cells 48 h postinfection in an inclusion with numerous surrounding mitochondria (arrow).

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