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Volume 11, Number 2—February 2005

Dispatch

Molecular Evidence of Pneumocystis Transmission in Pediatric Transplant Unit

Britta Höcker*Comments to Author , Constanze Wendt†, Aimable Nahimana‡, Burkhard Tönshoff*, and Philippe M. Hauser‡
Author affiliations: *University Children's Hospital, Heidelberg, Germany; †Hygiene Institute Heidelberg, Germany; ‡University Hospital of Lausanne, Lausanne, Switzerland

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Table

Pneumocystis jirovecii genotyping by PCR-SSCP of 4 genomic regions*

Patient Date SSCP pattern
ITS1 26S mt26 β-tub P. jirovecii type
Index case-patient 3/27/02 A A, B A, B,C A, B >2 types (nonidentifiable)
1 7/26/02 A A, B A A 1, 2
2 10/30/02 A A, B A A 1, 2
3 12/3/02 A A, B A A 1, 2
4 2/7/03 A, B A, B, C B, C B, C >2 types (nonidentifiable)
5 2/15/03 A A, B C A 6, 44
6 2/18/03 A, B A, B A C 45, 46

*Bold letters signify the most abundant simple pattern with the complex one, as shown by silver staining. Bold numbers signify the most abundant P. jirovecii type. PCR, polymerase chain reaction; SSCP, multitarget single-strand conformation polymorphism.

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