Volume 11, Number 4—April 2005
Letter
Route of Infection in Melioidosis
Jodie L. Barnes*
and Natkunam Ketheesan*
and Natkunam Ketheesan*Table
Ten-day LD50* values (given in CFU) after intravenous, intraperitoneal, subcutaneous, intranasal, or oral introduction of NCTC 13178 or NCTC 13179 strains of Burholderia pseudomallei into BALB/c or C57BL/6 mice
| Route of infection | NCTC 13178 |
NCTC 13179 |
||
|---|---|---|---|---|
| BALB/c | C57BL/6 | BALB/c | C57BL/6 | |
| Intravenous | <10 | 5 x 103 | 9 x 103 | 6 x 106 |
| Intraperitoneal | 1.2 x 101 | 9.7 x 103 | 4.7 x 105 | 2.1 x 107 |
| Subcutaneous | 1 x 103 | 9 x 105 | 9 x 102 | >108 |
| Intranasal† | 1.4 x 102 | 1.8 x 103 | 1.9 x 106 | >108 |
| Oral‡ | 7.2 x 103 | 1.8 106 | 4.8 x 106 | >108 |
*50% lethal dose.
†20 μL of challenge dose was introduced intranasally onto the nostrils of the mice by using a pipette tip.
‡20 μL of challenge dose was introduced orally to the back of the throat of the mice by using a pipette tip.
