Figure. A) Immunoblot of the patient (strip no. 21) and the patient's mother (strip no. 22) showing specific antibodies against 14- and 16-kDa proteins of Leishmania infantum. B) restriction fragment length polymorphism patterns after HaeIII digestion of the ribosomal internal transcribed spacer 1 polymerase chain reaction products. P, patient; Li, L. infantum; Lm, L. major; Lt, L. tropica; M, 100-bp ladder.