Appendix Figure. Molecular evidence for Borrelia infection. A) PCR for fla gene from Borrelia burgdorferi sensu lato; B) Southern blot assay with probes specific for B. burgdorferi sensu stricto; C) Sequences of fla gene amplified from 2 patients with erythema migrans (lines 1 and 2) and 1 with lymphocytoma (line 3) and aligned with the sequence of the fla gene from B. burgdorferi sensu stricto strain B31;and D) Sequence of the osp A gene amplified from a patient with erythema migrans (line 1) and aligned with the ospA gene from B. burgdorferi sensu stricto strain B31.
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