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Volume 13, Number 10—October 2007

Dispatch

Borrelia burgdorferi Infection and Cutaneous Lyme Disease, Mexico

Guadalupe Gordillo-Pérez*, Javier Torres*, Fortino Solórzano-Santos*, Sylvie de Martino†, Dan Lipsker†, Edmundo Velázquez*, Guillermo Ramon*, Muñoz Onofre*, and Benoit Jaulhac†
Author affiliations: *Instituto Mexicano del Seguro Social, Mexico City, Mexico; †Hôpitaux Universitaires, Strasbourg, France;

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Appendix Figure

Molecular evidence for Borrelia infection. A) PCR for fla gene from Borrelia burgdorferi sensu lato; B) Southern blot assay with probes specific for B. burgdorferi sensu stricto; C) Sequences of fla gene amplified from 2 patients with erythema migrans (lines 1 and 2) and 1 with lymphocytoma (line 3) and aligned with the sequence of the fla gene from B. burgdorferi sensu stricto strain B31;and D) Sequence of the osp A gene amplified from a patient with erythema migrans (line 1) and aligned with the ospA gene from B. burgdorferi sensu stricto strain B31.

Appendix Figure. Molecular evidence for Borrelia infection. A) PCR for fla gene from Borrelia burgdorferi sensu lato; B) Southern blot assay with probes specific for B. burgdorferi sensu stricto; C) Sequences of fla gene amplified from 2 patients with erythema migrans (lines 1 and 2) and 1 with lymphocytoma (line 3) and aligned with the sequence of the fla gene from B. burgdorferi sensu stricto strain B31;and D) Sequence of the osp A gene amplified from a patient with erythema migrans (line 1) and aligned with the ospA gene from B. burgdorferi sensu stricto strain B31.

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