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Volume 13, Number 10—October 2007

Dispatch

Novel Variant of Tickborne Encephalitis Virus, Russia

Vladimir A. Ternovoi*, Elena V. Protopopova*, Eugene V. Chausov*, Dmitry V. Novikov†, Galina N. Leonova‡, Sergey V. Netesov*, and Valery B. Loktev*Comments to Author 
Author affiliations: *State Research Center of Virology and Biotechnology “VECTOR,” Koltsovo, Novosibirsk Region, Russia; †Regional Clinical Hospital No. 1, Vladivostok, Russia; ‡Institute of Epidemiology and Microbiology, Vladivostok, Russia;

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Figure 2

Growth curves and E protein synthesis of Glubinnoe/2004 and 205 in pig kidney embryo (PKE) cells: monolayer of PKE cells were infected with Glubinnoe/2004 and 205 of tickborne encephalitis virus (TBEV). The cells were frozen and thawed 3× to release the virus, and infectious titers were determined by the viral cytopathic effect (in 50% tissue culture infective doses [TCID50]/mL–1) assay in PKE cells. ELISA using 10H10 and biotin-labeled EB1 anti–protein E monoclonal antibodies was used to determine the E protein levels in virus-infected cells. 1, Glubinnoe/2004, virus yield; 2, 205, virus yield; 3, Glubinnoe/2004, E protein synthesis; 4, 205, E protein synthesis. Bars represent mean ± SD.

Figure 2. Growth curves and E protein synthesis of Glubinnoe/2004 and 205 in pig kidney embryo (PKE) cells: monolayer of PKE cells were infected with Glubinnoe/2004 and 205 of tickborne encephalitis virus (TBEV). The cells were frozen and thawed 3× to release the virus, and infectious titers were determined by the viral cytopathic effect (in 50% tissue culture infective doses [TCID50]/mL–1) assay in PKE cells. ELISA using 10H10 and biotin-labeled EB1 anti–protein E monoclonal antibodies was used to determine the E protein levels in virus-infected cells. 1, Glubinnoe/2004, virus yield; 2, 205, virus yield; 3, Glubinnoe/2004, E protein synthesis; 4, 205, E protein synthesis. Bars represent mean ± SD.

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