Figure 2. Growth curves and E protein synthesis of Glubinnoe/2004 and 205 in pig kidney embryo (PKE) cells: monolayer of PKE cells were infected with Glubinnoe/2004 and 205 of tickborne encephalitis virus (TBEV). The cells were frozen and thawed 3× to release the virus, and infectious titers were determined by the viral cytopathic effect (in 50% tissue culture infective doses [TCID50]/mL–1) assay in PKE cells. ELISA using 10H10 and biotin-labeled EB1 anti–protein E monoclonal antibodies was used to determine the E protein levels in virus-infected cells. 1, Glubinnoe/2004, virus yield; 2, 205, virus yield; 3, Glubinnoe/2004, E protein synthesis; 4, 205, E protein synthesis. Bars represent mean ± SD.