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Volume 13, Number 10—October 2007

Dispatch

Rapid Field Immunoassay for Detecting Antibody to Sin Nombre Virus in Deer Mice

Tony Schountz*Comments to Author , Charles H. Calisher†, Tiffany R. Richens†, Audrey A. Rich*, Jeffrey B. Doty†, Mark T. Hughes†, and Barry J. Beaty†
Author affiliations: *University of Northern Colorado, Greeley, Colorado, USA; †Colorado State University, Fort Collins, Colorado, USA;

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Table

Comparison of results of PAGEIA and standard EIA for detection of antibody to Sin Nombre virus (SNV) in blood samples from 40 deer mice captured in southwest Colorado, 2006*

Accession no. Field PAGEIA
score† Laboratory PAGEIA 
OD‡ Laboratory PAGEIA
score§ Standard EIA 
OD¶ Standard EIA 
score#
HA-2548 3+ 1.254 Pos 0.903/0.066 Pos
HA-2552 4+ 2.406 Pos 1.083/0.113 Pos
HA-2554 4+ 1.788 Pos 1.395/0.058 Pos
HA-2558 4+ 2.383 Pos 1.462/0.055 Pos
HA-2560 4+ 1.913 Pos 1.378/0.086 Pos
HA-2564 0 0.001 Neg 0.327/0.055 Pos
HA-2565 1+ 0.236 Pos 0.715/0.046 Pos
HA-2567 4+ 2.123 Pos 1.485/0.080 Pos
HB-2604 4+ 2.065 Pos 1.161/0.067 Pos
HB-2608 2+ 0.855 Pos 0.653/0.095 Pos
HB-2612 1+ 0.282 Pos 1.136/0.071 Pos
HB-2616 1+ 0.311 Pos 0.458/0.001 Pos
HB-2617 2+ 0.517 Pos 0.819/0.008 Pos
HB-2618 2+ 0.494 Pos 1.085/0.009 Pos
HB-2622 3+ 1.254 Pos 1.519/0.029 Pos
HB-2628 1+ 0.493 Pos 0.220/0.082 Neg
HB-2630 3+ 1.609 Pos 0.681/0.008 Pos
HA-2570 4+ 1.970 Pos 0.389/0.024 Pos
HA-2578 4+ 2.101 Pos 1.185/0.017 Pos
HB-2642 4+ 2.784 Pos 1.294/0.063 Pos
HA-2601 4+ 2.699 Pos 0.921/0.121 Pos
HA-2609 2+ 0.608 Pos 0.228/0.042 Neg
HA-2612 4+ 2.482 Pos 1.072/0.085 Pos
HA-2616 1+ 0.331 Pos 0.076/0.059 Neg
HB-2681 3+ 0.977 Pos 1.392/0.048 Pos
HB-2682 3+ 1.095 Pos 1.326/0.042 Pos
HA-2634 4+ 3.010 Pos 0.863/0.014 Pos
HA-2647 4+ 2.824 Pos 0.720/0.023 Pos
HA-2666 4+ 2.682 Pos 0.477/0.028 Pos
HB-2706 1+ 0.836 Pos 0.324/0.032 Pos
HB-2710 2+ 0.664 Pos 0.155/0.035 Neg
HB-2712 3+ 1.599 Pos 0.345/0.033 Pos
HB-2717 1+ 1.098 Pos 0.293/0.027 Pos
HB-2720 3+ 2.581 Pos 0.630/0.039 Pos
TS-0830–6 2+ 0.889 Pos 0.799/0.097 Pos
TS0830–7 1+ 0.000 Neg 0.030/0.033 Neg
TS-0830–8 3+ 2.014 Pos 0.800/0.024 Pos
TS-0830–9 4+ 1.949 Pos 0.884/0.054 Pos
TS-0830–18 4+ 2.112 Pos 1.180/0.021 Pos
TS-0830–20 3+ 1.427 Pos 0.820/0.072 Pos

*PAGEIA, protein-A/G horseradish peroxidase enzyme-linked immunosorbent assay; EIA, enzyme immunoassay; OD, optical density; Pos, positive; Neg, negative.
†Field scores were based upon visual inspection without instrumentation, with 0 as negative, 1+ as very weak, 2+ as weak, 3+ as strong, and 4+ as very strong, relative to positive and negative control samples.
‡Field-collected samples were retested by PAGEIA under laboratory conditions and the OD reported here. The instrument was blanked on the negative control sample.
§OD >0.200 above the negative control was considered positive.
¶For laboratory EIA, OD was recorded for diluted (1:100) blood with both SNV antigen (numerator) and control antigen (denominator).
#A sample tested with SNV antigen and having an OD >0.300 was considered positive if the OD of that sample with the control antigen was <0.150. In regard to sample HBV-2717, the OD of the laboratory EIA with antigen was 0.293, very near the acceptable minimum, and the background was 0.027, which is very low; this sample was considered provisionally positive.

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