Protection and Virus Shedding of Falcons Vaccinated against Highly Pathogenic Avian Influenza A Virus (H5N1)
Michael Lierz* , Hafez M. Hafez*, Robert Klopfleisch†, Dörte Lüschow*, Christine Prusas*, Jens P. Teifke†, Miriam Rudolf†, Christian Grund†, Donata Kalthoff†, Thomas Mettenleiter†, Martin Beer†, and Timm Harder†
Author affiliations: *Freie Universität, Berlin, Germany; †Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany;
Figure 3. Detection of viral RNA by real-time reverse transcription–PCR (RT-PCR) from oropharyngeal (A) and cloacal (B) swabs of 5 vaccinated and 5 nonvaccinated falcons after challenge with 106.0 50% egg infectious doses of the highly pathogenic avian influenza virus strain A/Cygnus cygnus/Germany/R65/06 (H5N1). Y axis shows cycle-of-threshold (Ct) values of real-time RT-PCRs detecting an M gene fragment in individual swab samples of each animal. Asterisks represent extreme values; open circles show individual outliers; black bars within boxes indicate medians.
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