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Volume 13, Number 11—November 2007

Research

Protection and Virus Shedding of Falcons Vaccinated against Highly Pathogenic Avian Influenza A Virus (H5N1)

Michael Lierz*Comments to Author , Hafez M. Hafez*, Robert Klopfleisch†, Dörte Lüschow*, Christine Prusas*, Jens P. Teifke†, Miriam Rudolf†, Christian Grund†, Donata Kalthoff†, Thomas Mettenleiter†, Martin Beer†, and Timm Harder†
Author affiliations: *Freie Universität, Berlin, Germany; †Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany;

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Table 3

Viral RNA in tissues of 5 vaccinated and 5 nonvaccinated falcons*

Falcon no.,vaccination route RNA in tissue, ct value†
CNS Duodenum Pancreas Trachea Lung
Vaccinated
1, IM 36.12‡ >40.00 38.84‡ >40.00 >40.00
2, IM 38.65‡ >40.00 >40.00 >40.00 >40.00
5, IM 34.22‡ 36.14‡ >40.00 33.44‡§ 36.94‡
8, SC >40.00 IH >40.00 38.71‡ 32.85‡§
9, SC >40.00 IH IH 29.01§ IH
Nonvaccinated
11 18.22 25.84 19.70 19.09 20.75
12 23.79 23.37 14.67 19.07 18.95
13 12.85 26.22 20.13 19.82 16.95
14 11.52 21.66 17.14 16.70 15.55
15 14.13 16.31 19.91 14.61 18.04

*Viral RNA detected by real-time reverse transcription–PCR (RT-PCR) in vaccinated falcons euthanized 11 days postchallenge and in nonvaccinated falcons that died after challenge infection with 106.0 50% egg infectious doses of highly pathogenic avian influenza virus strain A/Cygnus cygnus/Germany/R65/06 (H5N1). Ct, cycle of threshold; CNS, central nervous system; IM, intramuscular; SC, subcutaneous; IH, inhibited (samples extracted twice with the QIAGEN [Hilden, Germany] Viral RNA kit or Trizol [Invitrogen, Carlsbad, CA, USA]).
†Real-time RT-PCR results are presented as ct values. Ct values >40 are scored as negative.
‡Virus isolation attempted in embryonated chicken eggs.
§Virus was isolated from individual tissue sample.

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